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  • Book
  • © 2003

Bone Research Protocols

Editors:

  1. Miep H. Helfrich

    1. University of Aberdeen Medical School, Aberdeen, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Stuart H. Ralston

    1. University of Aberdeen Medical School, Aberdeen, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 80)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Culture of Osteolblasts and Osteocytes

    1. Front Matter

      Pages 1-1
      PDF

    2. Human Osteoblast Culture

      • James A. Gallagher
      Pages 3-18

    3. Osteoblast Isolation from Murine Calvariae and Long Bones

      • Astrid Bakker, Jenneke Klein-Nulend
      Pages 19-28

    4. Mineralizing Fibroblast-Colony-Forming Assays

      • Andrew Scutt, Lynsey Reading, Nanette Scutt, Karen Still
      Pages 29-39

    5. Osteocyte Isolation and Culture

      • Peter J. Nijweide, Arie van der Plas, Marcel J. Alblas, Jenneke Klein-Nulend
      Pages 41-50

  3. Culture of Osteoclasts

    1. Front Matter

      Pages 51-51
      PDF

    2. Isolated Osteoclast Cultures

      • Astrid Hoebertz, Timothy R. Arnett
      Pages 53-64

    3. Primary Isolation and Culture of Chicken Osteoclasts

      • Patricia Collin-Osdoby, Fred Anderson, Philip Osdoby
      Pages 65-88

    4. Isolation and Purification of Rabbit Osteoclasts

      • Fraser P. Coxon, Julie C. Frith, Helena L. Benford, Michael J. Rogers
      Pages 89-99

    5. Generating Human Osteoclasts from Peripheral Blood

      • Afsie Sabokbar, Nicholas S. Athanasou
      Pages 101-111

    6. Generating Human Osteoclasts In Vitro from Bone Marrow and Peripheral Blood

      • Adrienne M. Flanagan, Helen M. Massey
      Pages 113-128

    7. Generating Murine Osteoclasts from Bone Marrow

      • Naoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka, Tatsuo Suda
      Pages 129-144

    8. Osteoclast Formation in the Mouse Coculture Assay

      • Robert J. van’t Hof
      Pages 145-152

    9. RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells

      • Patricia Collin-Osdoby, Xuefeng Yu, Hong Zheng, Philip Osdoby
      Pages 153-166

  4. Bone Organ Culture

    1. Front Matter

      Pages 167-167
      PDF

    2. Analysis of Osteoclast Function in Mouse Calvarial Cultures

      • Michael J. Marshall, Marit N. Rowlands
      Pages 169-182

  5. Histological and Ultrastructural Techniques

    1. Front Matter

      Pages 199-199
      PDF
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About this book

The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).
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Reviews

"Bone Research Protocols is a most welcome compilation of methods. In it, many of the most productive laboratories in some of the most challenging areas of cell biology reveal detailed protocols that have been used to solve key problems. Short of spending a sabbatical in one of the eminent laboratories that contributed, this volume provides the best possible introduction to these methods." - TRENDS in Endocrinology and Metabolism
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Editors and Affiliations

  • University of Aberdeen Medical School, Aberdeen, UK

    Miep H. Helfrich, Stuart H. Ralston

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Bibliographic Information

  • Book Title: Bone Research Protocols

  • Editors: Miep H. Helfrich, Stuart H. Ralston

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592593666

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Softcover ISBN: 978-1-61737-321-3Published: 09 November 2010

  • eBook ISBN: 978-1-59259-366-8Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 450

  • Topics: Orthopedics

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Buy it now

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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