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Stem Cell Technologies in Neuroscience

  • Book
  • © 2017

Overview

Editors:
  1. Amit K. Srivastava

    1. Department of Pediatric Surgery Children’s Program in Regenerative Medicine, University of Texas Health Science Center at Houston McGovern Medical School, Houston, USA

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  2. Evan Y. Snyder

    1. Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Yang D. Teng

    1. Departments of PM&R and Neurosurgery Harvard Medical School, Spaulding Rehabilitation Network and Brigham & Women’s Hospital, Boston, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Neuromethods (NM, volume 126)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Three-Dimensional Cultures of Human Neural Stem Cells: An Application for Modeling Alzheimer’s Disease Pathogenesis

    • Se Hoon Choi, Carla D’Avanzo, Young Hye Kim, Enjana Bylykbashi, Matthias Hebisch, Oliver Brüstle et al.
    Pages 1-18

  3. Neural Stem Cell Fate Control on Micropatterned Substrates

    • Leonora Buzanska, Marzena Zychowicz, Ana Ruiz, François Rossi
    Pages 19-44

  4. Dopaminergic and GABAergic Neuron In Vitro Differentiation from Embryonic Stem Cells

    • Talita Glaser, Juliana Corrêa-Velloso, Ágatha Oliveira-Giacomelli, Yang D. Teng, Henning Ulrich
    Pages 45-53

  5. Transfection of Cultured Primary Neurons

    • Annalisa Rossi, Ralf Dahm, Paolo Macchi
    Pages 55-78

  6. Reprogramming of Mouse Fibroblasts to Induced Oligodendrocyte Progenitor Cells

    • Robert T. Karl, Angela M. Lager, Fadi J. Najm, Paul J. Tesar
    Pages 79-93

  7. Isolation and Culture of Oligodendrocyte Precursor Cells from Prenatal and Postnatal Rodent Brain

    • Danyang He, Bradley Meyer, Q. Richard Lu
    Pages 95-109

  8. Schwann Cell Isolation and Culture Reveals the Plasticity of These Glia

    • David E. Weinstein
    Pages 111-121

  9. Generation of Cerebral Organoids Derived from Human Pluripotent Stem Cells

    • Mark E. Hester, Alexis B. Hood
    Pages 123-134

  10. Development of Mouse Cell-Based In Vitro Blood-Brain Barrier Models

    • Malgorzata Burek, Ellaine Salvador, Carola Y. Förster
    Pages 135-143

  11. Microfluidic Device for Studying Traumatic Brain Injury

    • Yiing Chiing Yap, Tracey C. Dickson, Anna E. King, Michael C. Breadmore, Rosanne M. Guijt
    Pages 145-156

  12. Multimodal Neural Stem Cell Research Protocols for Experimental Spinal Cord Injuries

    • Yang D. Teng, Evan Y. Snyder, Xiang Zeng, Liquan Wu, Inbo Han
    Pages 157-173

  13. Real-Time Dual MRI for Predicting and Subsequent Validation of Intra-Arterial Stem Cell Delivery to the Central Nervous System

    • Piotr Walczak, Miroslaw Janowski
    Pages 175-191

  14. Standardized Cryopreservation of Stem Cells

    • Maria L. Thompson, Eric J. Kunkel, Rolf O. Ehrhardt
    Pages 193-203

  15. Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies

    • Zezhou Zhao, Dillon C. Muth, Vasiliki Mahairaki, Linzhao Cheng, Kenneth W. Witwer
    Pages 205-223

  16. Essential Requirements for Setting Up a Stem Cell Laboratory

    • Philip H. Schwartz, Robin L. Wesselschmidt
    Pages 225-237

  17. Back Matter

    Pages 239-240
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Keywords

About this book

This volume provides comprehensive knowledge on the fundamental techniques and detailed laboratory protocols used in stem cell research and their potential application in the field of neuroscience. The chapters in this book cover detailed descriptions of techniques for isolation, maintenance, differentiation, and characterization of neural stem cells, and the development of in vitro stem cell-based neurological disease model. There are detailed methods on leading techniques for stem cell genetic engineering and intra-organ transplantation for research and therapeutic purposes, in vivo imaging of donor stem cells, and essential requirements for setting up a new stem cell laboratory. Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and pragmatic, Stem Cell Technologies in Neuroscience is a valuable resource for academic and translational researchers, industry scientists, biology teachers, and life science graduate students to grasp the vital pulse of the rapidly growing field of stem cell research.

 

Editors and Affiliations

  • Department of Pediatric Surgery Children’s Program in Regenerative Medicine, University of Texas Health Science Center at Houston McGovern Medical School, Houston, USA

    Amit K. Srivastava

  • Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA

    Evan Y. Snyder

  • Departments of PM&R and Neurosurgery Harvard Medical School, Spaulding Rehabilitation Network and Brigham & Women’s Hospital, Boston, USA

    Yang D. Teng

Bibliographic Information

  • Book Title: Stem Cell Technologies in Neuroscience

  • Editors: Amit K. Srivastava, Evan Y. Snyder, Yang D. Teng

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7024-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7022-3Published: 12 May 2017

  • Softcover ISBN: 978-1-4939-8371-1Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7024-7Published: 10 May 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 240

  • Number of Illustrations: 9 b/w illustrations, 46 illustrations in colour

  • Topics: Neurosciences

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