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Haplotyping

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Irene Tiemann-Boege

    1. Johannes Kepler University Institute of Biophysics, Linz, Austria

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    You can also search for this editor in PubMed   Google Scholar

  2. Andrea Betancourt

    1. Vetmeduni Vienna InstitutPopulationsgenetik, Wien, Austria

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1551)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
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  2. Haplotyping with Long Range PCR

    1. Front Matter

      Pages 1-1
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    2. Haplotyping of Heterozygous SNPs in Genomic DNA Using Long-Range PCR

      • Barbara Arbeithuber, Angelika Heissl, Irene Tiemann-Boege
      Pages 3-22

    3. Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA

      • Kyuha Choi, Nataliya E. Yelina, Heïdi Serra, Ian R. Henderson
      Pages 23-57

  3. Selective Sequencing of Gene Families (including the MHC)

    1. Front Matter

      Pages 59-59
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    2. PacBio for Haplotyping in Gene Families

      • Wei Zhang, Joachim Messing
      Pages 61-71

    3. High Molecular Weight DNA Enrichment with Peptide Nucleic Acid Probes

      • Nicholas M. Murphy, Colin W. Pouton, Helen R. Irving
      Pages 73-85

    4. High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture

      • Johannes Pröll, Carina Fischer, Gabriele Michelitsch, Martin Danzer, Norbert Niklas
      Pages 87-112

    5. Pedigree-Defined Haplotypes and Their Applications to Genetic Studies

      • Chester A. Alper, Charles E. Larsen
      Pages 113-127

  5. Haplotyping Single Chromosomes

    1. Front Matter

      Pages 159-159
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    2. Chromosome-Range Whole-Genome High-Throughput Experimental Haplotyping by Single-Chromosome Microdissection

      • Li Ma, Wenzhi Li, Qing Song
      Pages 161-169

    3. Phased Genome Sequencing Through Chromosome Sorting

      • Xi Chen, Hong Yang, Wing Hung Wong
      Pages 171-188

  6. Genome Wide Haplotyping

    1. Front Matter

      Pages 189-189
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    2. Long Fragment Read (LFR) Technology: Cost-Effective, High-Quality Genome-Wide Molecular Haplotyping

      • Mark A. McElwain, Rebecca Yu Zhang, Radoje Drmanac, Brock A. Peters
      Pages 191-205

    3. Contiguity-Preserving Transposition Sequencing (CPT-Seq) for Genome-Wide Haplotyping, Assembly, and Single-Cell ATAC-Seq

      • Lena Christiansen, Sasan Amini, Fan Zhang, Mostafa Ronaghi, Kevin L. Gunderson, Frank J. Steemers
      Pages 207-221

    4. A Fosmid Pool-Based Next Generation Sequencing Approach to Haplotype-Resolve Whole Genomes

      • Eun-Kyung Suk, Sabrina Schulz, Birgit Mentrup, Thomas Huebsch, Jorge Duitama, Margret R. Hoehe
      Pages 223-269

  7. High throughput Haplotyping of Rare Variants

    1. Front Matter

      Pages 271-271
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Keywords

About this book

This volume details protocols for genetic, molecular, cytological, and bioinformatic methods for determining haplotypes.  Haplotyping: Methods and Protocols guides readers through methods that directly type haploid cells, difficult-to-resolve gene families, high-resolution, short range haplotyping for targeted loci, and long-range haplotyping for whole chromosomes or genomes.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical Haplotyping: Methods and Protocols, aims to provide researchers with an overview of experimental methods for haplotyping.

Editors and Affiliations

  • Johannes Kepler University Institute of Biophysics, Linz, Austria

    Irene Tiemann-Boege

  • Vetmeduni Vienna InstitutPopulationsgenetik, Wien, Austria

    Andrea Betancourt

Bibliographic Information

  • Book Title: Haplotyping

  • Book Subtitle: Methods and Protocols

  • Editors: Irene Tiemann-Boege, Andrea Betancourt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6750-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6748-3Published: 30 January 2017

  • Softcover ISBN: 978-1-4939-8291-2Published: 15 July 2018

  • eBook ISBN: 978-1-4939-6750-6Published: 20 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 326

  • Number of Illustrations: 17 b/w illustrations, 37 illustrations in colour

  • Topics: Human Genetics

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