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Oncogene-Induced Senescence

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Mikhail A. Nikiforov

    1. Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, USA

    View editor publications

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  • Includes cutting-edge methods and protocols for the study of oncogene-induced senescence
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1534)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Immortal Senescence

    • Anna Bianchi-Smiraglia, Brittany C. Lipchick, Mikhail A. Nikiforov
    Pages 1-15

  3. Senescence Phenotypes Induced by Ras in Primary Cells

    • Lena Lau, Gregory David
    Pages 17-30

  4. Cellular Model of p21-Induced Senescence

    • Michael Shtutman, Bey-Dih Chang, Gary P. Schools, Eugenia V. Broude
    Pages 31-39

  5. Detecting Markers of Therapy-Induced Senescence in Cancer Cells

    • Dorothy N. Y. Fan, Clemens A. Schmitt
    Pages 41-52

  6. Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence

    • Maria V. Guijarro, Amancio Carnero
    Pages 53-68

  7. Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence

    • Priyanka L. Patel, Utz Herbig
    Pages 69-78

  8. RT-qPCR Detection of Senescence-Associated Circular RNAs

    • Amaresh C. Panda, Kotb Abdelmohsen, Myriam Gorospe
    Pages 79-87

  9. Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy

    • Masako Narita, Masashi Narita
    Pages 89-98

  10. Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis

    • Priya Hari, Juan Carlos Acosta
    Pages 99-109

  11. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells

    • Konstantinos Evangelou, Vassilis G. Gorgoulis
    Pages 111-119

  12. Using [U-13C6]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts

    • Katerina I. Leonova, David A. Scott
    Pages 121-125

  13. Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence

    • Hengrui Zhu, Linh Le, Hsin-Yao Tang, David W. Speicher, Rugang Zhang
    Pages 127-137

  14. Detection of Reactive Oxygen Species in Cells Undergoing Oncogene-Induced Senescence

    • Rabii Ameziane-El-Hassani, Corinne Dupuy
    Pages 139-145

  15. Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach

    • Mohammad Althubiti, Salvador Macip
    Pages 147-153

  16. Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence

    • Claudia Gey, Karsten Seeger
    Pages 155-163

  17. Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence

    • Mikhail A. Nikiforov, Donna S. Shewach
    Pages 165-173

  18. Senescence-Like Phenotypes in Human Nevi

    • Andrew Joselow, Darren Lynn, Tamara Terzian, Neil F. Box
    Pages 175-184

  19. Detection of Oncogene-Induced Senescence In Vivo

    • Kwan-Hyuck Baek, Sandra Ryeom
    Pages 185-198

  20. Detection of Senescence Markers During Mammalian Embryonic Development

    • Mekayla Storer, William M. Keyes
    Pages 199-210
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Keywords

About this book

This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster, as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.

Editors and Affiliations

  • Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, USA

    Mikhail A. Nikiforov

Bibliographic Information

  • Book Title: Oncogene-Induced Senescence

  • Book Subtitle: Methods and Protocols

  • Editors: Mikhail A. Nikiforov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6670-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6668-4Published: 03 November 2016

  • Softcover ISBN: 978-1-4939-8267-7Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6670-7Published: 03 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 220

  • Number of Illustrations: 9 b/w illustrations, 23 illustrations in colour

  • Topics: Cancer Research

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