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  • © 2017

Hypertension

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1527)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xv
  2. Large-Scale Transcriptome Analysis

    • David Weaver, Kathirvel Gopalakrishnan, Bina Joe
    Pages 1-26
  3. Methods to Assess Genetic Risk Prediction

    • Christin Schulz, Sandosh Padmanabhan
    Pages 27-40
  4. Microarray Analysis of Hypertension

    • Henry L. Keen, Curt D. Sigmund
    Pages 41-52
  5. Tissue Proteomics in Vascular Disease

    • Amaya Albalat, William Mullen, Holger Husi, Harald Mischak
    Pages 53-60
  6. Urine Metabolomics in Hypertension Research

    • Sofia Tsiropoulou, Martin McBride, Sandosh Padmanabhan
    Pages 61-68
  7. Systems Biology Approach in Hypertension Research

    • Christian Delles, Holger Husi
    Pages 69-79
  8. Measurement of Angiotensin Peptides: HPLC-RIA

    • K. Bridget Brosnihan, Mark C. Chappell
    Pages 81-99
  9. Analysis of the Aldosterone Synthase (CYP11B2) and 11β-Hydroxylase (CYP11B1) Genes

    • Scott M. MacKenzie, Eleanor Davies, Samantha Alvarez-Madrazo
    Pages 139-150
  10. Dopaminergic Immunofluorescence Studies in Kidney Tissue

    • J. J. Gildea, R. E. Van Sciver, H. E. McGrath, B. A. Kemp, P. A. Jose, R. M. Carey et al.
    Pages 151-161
  11. Intracellular Free Calcium Measurement Using Confocal Imaging

    • Ghassan Bkaily, Johny Al-Khoury, Yanick Simon, Danielle Jacques
    Pages 177-187
  12. In Vitro and In Vivo Approaches to Assess Rho Kinase Activity

    • Vincent Sauzeau, Gervaise Loirand
    Pages 213-218
  13. NADPH Oxidases and Measurement of Reactive Oxygen Species

    • Angelica Amanso, Alicia N. Lyle, Kathy K. Griendling
    Pages 219-232
  14. Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation

    • Denise C. Fernandes, Renata C. Gonçalves, Francisco R. M. Laurindo
    Pages 233-249

About this book

This volume provides comprehensive protocols that researchers can use to dissect out fundamental principles and molecular mechanisms of hypertension, extending from genetics of experimental hypertension to biomarkers in clinical hypertension. Hypertension: Methods and Protocols guides readers through chapters on genetics and mmics of hypertension; the Renin-Angiotensin-Aldosterone System; vasoactive agents and hypertension; signal transduction and reactive oxygen species; novel cell models and approaches to study molecular mechanisms of hypertension; vascular physiology; and new approaches to manipulate mouse models to study molecular mechanisms of hypertension. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

  

Authoritative and cutting-edge, Hypertension: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  • Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

Bibliographic Information

  • Book Title: Hypertension

  • Book Subtitle: Methods and Protocols

  • Editors: Rhian M. Touyz, Ernesto L. Schiffrin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6625-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6623-3Published: 24 January 2017

  • Softcover ISBN: 978-1-4939-8253-0Published: 15 July 2018

  • eBook ISBN: 978-1-4939-6625-7Published: 23 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 437

  • Number of Illustrations: 38 b/w illustrations, 40 illustrations in colour

  • Topics: Molecular Medicine

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access