Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1515)
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Table of contents (16 protocols)
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Front Matter
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In Vitro and In Vivo Systems to Dissect Molecular Mechanisms of Cohesin Regulation in Mitosis and Meiosis
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Front Matter
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The Roles of Cohesin in Gene Regulation, Chromatin Interactions, Ribosome Biogenesis, and DNA Repair
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Front Matter
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Dissection of Condensin Function in Mitotic Chromosome Condensation and Gene Regulation
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Front Matter
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Keywords
About this book
The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.
Editors and Affiliations
Bibliographic Information
Book Title: Cohesin and Condensin
Book Subtitle: Methods and Protocols
Editors: Kyoko Yokomori, Katsuhiko Shirahige
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6545-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6543-4Published: 29 October 2016
Softcover ISBN: 978-1-4939-8232-5Published: 06 July 2018
eBook ISBN: 978-1-4939-6545-8Published: 28 October 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 276
Number of Illustrations: 8 b/w illustrations, 33 illustrations in colour
Topics: Cell Biology