Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1509)
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Table of contents (21 protocols)
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Front Matter
About this book
This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.
Editors and Affiliations
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Department of Science, Waterford Institute of Technology, Waterford, Ireland
Sweta Rani
Bibliographic Information
Book Title: MicroRNA Profiling
Book Subtitle: Methods and Protocols
Editors: Sweta Rani
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6524-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6522-9Published: 09 November 2016
Softcover ISBN: 978-1-4939-8225-7Published: 20 June 2018
eBook ISBN: 978-1-4939-6524-3Published: 08 November 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 246
Number of Illustrations: 9 b/w illustrations, 32 illustrations in colour
Topics: Human Genetics