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The Nucleolus

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Attila Németh

    1. Department of Biochemistry III, University of Regensburg, Regensburg, Germany

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1455)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Imaging of the Nucleolus

    1. Front Matter

      Pages 1-1
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    2. The Relationship Between Human Nucleolar Organizer Regions and Nucleoli, Probed by 3D-ImmunoFISH

      • Marjolein van Sluis, Chelly van Vuuren, Brian McStay
      Pages 3-14

    3. Analysis of the C. elegans Nucleolus by Immuno-DNA FISH

      • Christian Lanctôt
      Pages 15-27

    4. Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae

      • Christophe Normand, Maxime Berthaud, Olivier Gadal, Isabelle Léger-Silvestre
      Pages 29-40

    5. High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae

      • Renjie Wang, Christophe Normand, Olivier Gadal
      Pages 41-57

    6. Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin

      • Stefan Dillinger, Attila Németh
      Pages 59-69

    7. Visualization of the Nucleolus in Living Cells with Cell-Penetrating Fluorescent Peptides

      • Robert M. Martin, Henry D. Herce, Anne K. Ludwig, M. Cristina Cardoso
      Pages 71-82

  3. Analysis of Ribosomal RNA Transcription and Processing

    1. Front Matter

      Pages 83-83
      Download chapter PDF

    2. Purification of Crystallization-Grade RNA Polymerase I from S. cerevisiae

      • Christoph Engel
      Pages 85-97

    3. Analysis of S. cerevisiae RNA Polymerase I Transcription In Vitro

      • Michael Pilsl, Philipp E. Merkl, Philipp Milkereit, Joachim Griesenbeck, Herbert Tschochner
      Pages 99-108

    4. Preparation of Chromatin Templates to Study RNA Polymerase I Transcription In Vitro

      • Gernot Längst
      Pages 109-119

    5. A Nonradioactive Assay to Measure Production and Processing of Ribosomal RNA by 4sU-Tagging

      • Kaspar Burger, Dirk Eick
      Pages 121-131

    6. Metabolic Labeling in the Study of Mammalian Ribosomal RNA Synthesis

      • Victor Y. Stefanovsky, Tom Moss
      Pages 133-145

    7. Quantitative Northern Blot Analysis of Mammalian rRNA Processing

      • Minshi Wang, Dimitri G. Pestov
      Pages 147-157

  4. Genomics and Proteomics of the Nucleolus

    1. Front Matter

      Pages 159-159
      Download chapter PDF

    2. Complete Sequence Construction of the Highly Repetitive Ribosomal RNA Gene Repeats in Eukaryotes Using Whole Genome Sequence Data

      • Saumya Agrawal, Austen R. D. Ganley
      Pages 161-181

    3. Analysis of rRNA Gene Methylation in Arabidopsis thaliana by CHEF-Conventional 2D Gel Electrophoresis

      • Gireesha Mohannath, Craig S. Pikaard
      Pages 183-202

    4. Fluorescence-Activated Nucleolus Sorting in Arabidopsis

      • Frédéric Pontvianne, Myriam Boyer-Clavel, Julio Sáez-Vásquez
      Pages 203-211

    5. Purification of RNA Polymerase I-Associated Chromatin from Yeast Cells

      • Astrid Bruckmann, Jan Linnemann, Jorge Perez-Fernandez
      Pages 213-223
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Keywords

About this book

This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing, and genomics and proteomics of the nucleolus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical handbook to facilitate the investigation of this nuclear compartment at the advanced level.

Editors and Affiliations

  • Department of Biochemistry III, University of Regensburg, Regensburg, Germany

    Attila Németh

Bibliographic Information

  • Book Title: The Nucleolus

  • Book Subtitle: Methods and Protocols

  • Editors: Attila Németh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3792-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3790-5Published: 31 August 2016

  • Softcover ISBN: 978-1-4939-8140-3Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3792-9Published: 30 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 279

  • Number of Illustrations: 12 b/w illustrations, 43 illustrations in colour

  • Topics: Cell Biology

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