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  • © 2016

Mouse Models for Drug Discovery

Methods and Protocols

  • Incudes fully updated cutting-edge methods and protocols for using mouse models in drug research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1438)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Simple, Efficient CRISPR-Cas9-Mediated Gene Editing in Mice: Strategies and Methods

    • Benjamin E. Low, Peter M. Kutny, Michael V. Wiles
    Pages 19-53
  3. Mouse Sperm Cryopreservation and Recovery of Genetically Modified Mice

    • Benjamin E. Low, Rob A. Taft, Michael V. Wiles
    Pages 55-66
  4. Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftment of Hematopoietic Stem Cells

    • Suheyla Hasgur, Ken Edwin Aryee, Leonard D. Shultz, Dale L. Greiner, Michael A. Brehm
    Pages 67-78
  5. Generation of Human Liver Chimeric Mice for the Study of Human Hepatotropic Pathogens

    • Markus von Schaewen, Gabriela Hrebikova, Alexander Ploss
    Pages 79-101
  6. Human FcRn Transgenic Mice for Pharmacokinetic Evaluation of Therapeutic Antibodies

    • Derry C. Roopenian, Gregory J. Christianson, Gabriele Proetzel, Thomas J. Sproule
    Pages 103-114
  7. Germ-Free Mice Model for Studying Host–Microbial Interactions

    • Yogesh Bhattarai, Purna C. Kashyap
    Pages 123-135
  8. Cholesterol Absorption and Metabolism

    • Philip N. Howles
    Pages 177-197
  9. Skin Diseases in Laboratory Mice: Approaches to Drug Target Identification and Efficacy Screening

    • John P. Sundberg, Kathleen A. Silva, Lloyd E. King Jr, C. Herbert Pratt
    Pages 199-224
  10. Murine Model for Colitis-Associated Cancer of the Colon

    • Ashley J. Snider, Agnieszka B. Bialkowska, Amr M. Ghaleb, Vincent W. Yang, Lina M. Obeid, Yusuf A. Hannun
    Pages 245-254
  11. Mouse Models for Studying Depression-Like States and Antidepressant Drugs

    • Carisa L. Bergner, Amanda N. Smolinsky, Peter C. Hart, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte et al.
    Pages 255-269
  12. Experimental Models of Anxiety for Drug Discovery and Brain Research

    • Peter C. Hart, Carisa L. Bergner, Amanda N. Smolinsky, Brett D. Dufour, Rupert J. Egan, Justin L. LaPorte et al.
    Pages 271-291
  13. Better Utilization of Mouse Models of Neurodegenerative Diseases in Preclinical Studies: From the Bench to the Clinic

    • Christopher Janus, Carolina Hernandez, Victoria deLelys, Hanno Roder, Hans Welzl
    Pages 311-347
  14. Neuromuscular Disease Models and Analysis

    • Robert W. Burgess, Gregory A. Cox, Kevin L. Seburn
    Pages 349-394

About this book

With genetic engineering, systems explored in this book now exist allowing for the simple, efficient, and near universally precise genetic manipulation directly in any organism, including the mouse. Herein, these models are applied to a wide field of disease areas, including diabetes, cardiovascular disease, skin disorders, cancer, neurodegenerative and neuromuscular diseases, retinal disorders, as well as various behavioral models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and fully updated, Mouse Models for Drug Discovery: Methods and Protocols, Second Edition serves to equip the reader with an extensive overview of techniques to utilize the many possibilities of mice in the drug development process.

Editors and Affiliations

  • Takeda Pharmaceutical Company, Cambridge, USA

    Gabriele Proetzel

  • The Jackson Laboratory, Bar Harbor, USA

    Michael V. Wiles

Bibliographic Information

  • Book Title: Mouse Models for Drug Discovery

  • Book Subtitle: Methods and Protocols

  • Editors: Gabriele Proetzel, Michael V. Wiles

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3661-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3659-5Published: 06 May 2016

  • Softcover ISBN: 978-1-4939-8109-0Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3661-8Published: 05 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 436

  • Number of Illustrations: 26 b/w illustrations, 37 illustrations in colour

  • Topics: Pharmacology/Toxicology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access