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Yeast Cytokinesis

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Alberto Sanchez-Diaz

    1. Departamento de Biología Molecular, Universidad de Cantabria, Facultad de Medicina, Cardenal Herrera Oria s/n, Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria, CSIC, Santander, Spain

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  2. Pilar Perez

    1. Universidad de Salamanca, Instituto de Biología Funcional y Genómica, CSIC, Salamanca, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1369)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Time-Lapse Fluorescence Microscopy of Budding Yeast Cells

    • Arun Kumar, Manuel Mendoza
    Pages 1-8

  3. Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells

    • Reshma Davidson, Yajun Liu, Kenneth S. Gerien, Jian-Qiu Wu
    Pages 9-23

  4. Fluorescence Recovery After Photo-Bleaching (FRAP) and Fluorescence Loss in Photo-Bleaching (FLIP) Experiments to Study Protein Dynamics During Budding Yeast Cell Division

    • Alessio Bolognesi, Andrzej Sliwa-Gonzalez, Rupali Prasad, Yves Barral
    Pages 25-44

  5. High-Speed Super-Resolution Imaging of Live Fission Yeast Cells

    • Caroline Laplante, Fang Huang, Joerg Bewersdorf, Thomas D. Pollard
    Pages 45-57

  6. Monitoring Chitin Deposition During Septum Assembly in Budding Yeast

    • Irene Arcones, Cesar Roncero
    Pages 59-72

  7. Imaging Septum Formation by Fluorescence Microscopy

    • Juan Carlos Ribas, Juan Carlos G. Cortés
    Pages 73-85

  8. Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy

    • Franz Meitinger, Gislene Pereira
    Pages 87-95

  9. Visualization of Fission Yeast Cells by Transmission Electron Microscopy

    • Matthias Sipiczki
    Pages 97-111

  10. Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography

    • Aurélie Bertin, Eva Nogales
    Pages 113-123

  11. Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe

    • Junqi Huang, Mithilesh Mishra, Saravanan Palani, Ting Gang Chew, Mohan K. Balasubramanian
    Pages 125-136

  12. Measurements of Myosin-II Motor Activity During Cytokinesis in Fission Yeast

    • Qing Tang, Luther W. Pollard, Matthew Lord
    Pages 137-150

  13. In Vitro Biochemical Characterization of Cytokinesis Actin-Binding Proteins

    • Dennis Zimmermann, Alisha N. Morganthaler, David R. Kovar, Cristian Suarez
    Pages 151-179

  14. Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins

    • Nathan A. McDonald, Kathleen L. Gould
    Pages 181-189

  15. Analysis of Three-Dimensional Structures of Exocyst Components

    • Johannes Lesigang, Gang Dong
    Pages 191-204

  16. Analysis of Rho-GTPase Activity During Budding Yeast Cytokinesis

    • Masayuki Onishi, John R. Pringle
    Pages 205-218

  17. Detection of Phosphorylation Status of Cytokinetic Components

    • Franz Meitinger, Saravanan Palani, Gislene Pereira
    Pages 219-237

  18. Studying Protein–Protein Interactions in Budding Yeast Using Co-immunoprecipitation

    • Magdalena Foltman, Alberto Sanchez-Diaz
    Pages 239-256

  19. Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes

    • Asli Devrekanli, Masato T. Kanemaki
    Pages 257-278

  20. Synchronization of the Budding Yeast Saccharomyces cerevisiae

    • Magdalena Foltman, Iago Molist, Alberto Sanchez-Diaz
    Pages 279-291
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Keywords

About this book

This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.

Editors and Affiliations

  • Departamento de Biología Molecular, Universidad de Cantabria, Facultad de Medicina, Cardenal Herrera Oria s/n, Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria, CSIC, Santander, Spain

    Alberto Sanchez-Diaz

  • Universidad de Salamanca, Instituto de Biología Funcional y Genómica, CSIC, Salamanca, Spain

    Pilar Perez

Bibliographic Information

  • Book Title: Yeast Cytokinesis

  • Book Subtitle: Methods and Protocols

  • Editors: Alberto Sanchez-Diaz, Pilar Perez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3145-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3144-6Published: 09 November 2015

  • Softcover ISBN: 978-1-4939-7989-9Published: 30 March 2019

  • eBook ISBN: 978-1-4939-3145-3Published: 30 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 395

  • Number of Illustrations: 31 b/w illustrations, 52 illustrations in colour

  • Topics: Cell Biology

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