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Immunosenescence

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Albert C. Shaw

    1. Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1343)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation

    • Carl Fortin, Tamas Fülöp
    Pages 1-7

  3. Flow Cytometry Analysis of NK Cell Phenotype and Function in Aging

    • Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa, Rafael Solana
    Pages 9-18

  4. Flow Cytometric Identification of Fibrocytes in the Human Circulation

    • Xinyuan Hu, Erin M. DeBiasi, Erica L. Herzog
    Pages 19-33

  5. Experimental Approaches to Tissue Injury and Repair in Advanced Age

    • Aleah L. Brubaker, Stewart R. Carter, Elizabeth J. Kovacs
    Pages 35-51

  6. Multicolor Digital Flow Cytometry in Human Translational Immunology

    • Samit R. Joshi, Subhasis Mohanty, Albert C. Shaw
    Pages 53-64

  7. Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates

    • Christine Meyer, Kristen Haberthur, Mark Asquith, Ilhem Messaoudi
    Pages 65-80

  8. Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry

    • Michael D. Leipold, Evan W. Newell, Holden T. Maecker
    Pages 81-95

  9. Imaging Immunosenescence

    • Feng Qian, Ruth R. Montgomery
    Pages 97-106

  10. Activation-Induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine

    • Daniela Frasca, Alain Diaz, Bonnie B. Blomberg
    Pages 107-114

  11. Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry

    • Min Sun Shin, Insoo Kang
    Pages 115-119

  12. Cell-Mediated Immune Response to Influenza Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses

    • Janet E. McElhaney, Beth Gentleman
    Pages 121-141

  13. Assays for Monitoring Macroautophagy Activity in T cells

    • Yair Botbol, Fernando Macian
    Pages 143-153

  14. Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides, and Protein Conformation in Biological Tissues

    • Asish R. Chaudhuri, Rochelle Wei, Arunabh Bhattacharya, Ryan Hamilton
    Pages 155-173

  15. Monitoring the DNA Damage Response at Dysfunctional Telomeres

    • Rekha Rai, Sandy Chang
    Pages 175-180

  16. Single-Cell Analysis of T-Cell Receptor αβ Repertoire

    • Pradyot Dash, George C. Wang, Paul G. Thomas
    Pages 181-197

  17. Assessment of B Cell Repertoire in Humans

    • Yu-Chang Wu, David Kipling, Deborah Dunn-Walters
    Pages 199-218

  18. Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-Throughput DNA Sequencing

    • Chen Wang, Yi Liu, Krishna M. Roskin, Katherine J. L. Jackson, Scott D. Boyd
    Pages 219-233

  19. Discovery of Novel microRNAs in Aging Caenorhabditis elegans

    • Alexandre de Lencastre, Frank Slack
    Pages 235-248

  20. Analysis of DNA Methylation by Pyrosequencing

    • Colin Delaney, Sanjay K. Garg, Raymond Yung
    Pages 249-264
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Keywords

About this book

In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.

Editors and Affiliations

  • Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, USA

    Albert C. Shaw

Bibliographic Information

  • Book Title: Immunosenescence

  • Book Subtitle: Methods and Protocols

  • Editors: Albert C. Shaw

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2963-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media 2015

  • Hardcover ISBN: 978-1-4939-2962-7Published: 30 September 2015

  • Softcover ISBN: 978-1-4939-4418-7Published: 29 October 2016

  • eBook ISBN: 978-1-4939-2963-4Published: 29 September 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 270

  • Number of Illustrations: 22 b/w illustrations, 27 illustrations in colour

  • Topics: Immunology

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