Ionotropic Glutamate Receptor Technologies

1. Front Matter

   Pages i-xii

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2. Protein Assembly and Trafficking

   1. Front Matter

      Pages 1-1

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   2. Assaying AMPA Receptor Oligomerization

      • Catherine L. Salussolia, Quan Gan, Lonnie P. Wollmuth

      Pages 3-14

   3. A Step-by-Step Guide to Single-Subunit Counting of Membrane-Bound Proteins in Mammalian Cells

      • Mark R. P. Aurousseau, Hugo McGuire, Rikard Blunck, Derek Bowie

      Pages 15-30

   4. Counting NMDA Receptors at the Cell Surface

      • Martin Horak, Young Ho Suh

      Pages 31-44

   5. Electrophysiological Tagging of Ionotropic Glutamate Receptors

      • Andres Barria

      Pages 45-60

   6. Electron Microscopy Analysis of AMPA Receptors in Dendritic Spines

      • Audra A. Kramer, Amber N. Petersen, Nashaat Z. Gerges

      Pages 61-69

   7. Functional Detection of Novel Triheteromeric NMDA Receptors

      • Sanjay S. Kumar

      Pages 71-80

3. Protein Structure and Dynamics

   1. Front Matter

      Pages 81-81

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   2. Expression, Purification, and Crystallization of Full Length Ionotropic Glutamate Receptors

      • Maria V. Yelshanskaya, Kei Saotome, Minfen Li, Alexander I. Sobolevsky

      Pages 83-100

   3. NMR Approaches to Functional Dynamics of Genetically Separated iGluR Domains

      • Christopher P. Ptak, Ahmed H. Ahmed, Robert E. Oswald

      Pages 101-118

   4. Computing Conformational Free Energies of iGluR Ligand-Binding Domains

      • Alvin Yu, Tyler Wied, John Belcher, Albert Y. Lau

      Pages 119-132

   5. LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors

      • Rita E. Sirrieh, Vasanthi Jayaraman

      Pages 133-144

   6. Assaying the Energetics of NMDA Receptor Pore Opening

      • Rashek Kazi, Melissa Daniel, Lonnie P. Wollmuth

      Pages 145-162

4. Protein Function

   1. Front Matter

      Pages 163-163

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   2. Constructing a Rapid Solution Exchange System

      • David M. MacLean

      Pages 165-183

   3. Assessing The Effects of Ligand-Binding Mutations to AMPA and Kainate Receptor Kinetics

      • Mark W. Fleck

      Pages 185-203

   4. Analysis of Whole-Cell NMDA Receptor Currents

      • Vojtech Vyklicky, Miloslav Korinek, Ales Balik, Tereza Smejkalova, Barbora Krausova, Ladislav Vyklicky

      Pages 205-219

   5. Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses

      • Kelly A. Krogh, Stanley A. Thayer

      Pages 221-239

   6. Timing AMPA Receptor Activation with Laser-Pulse Photolysis

      • Li Niu

      Pages 241-255

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology.  Chapters in this book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations.

• atomic organization
• iGluR physiology
• iGluR function
• iGluR structure
• intramolecular motions
• receptor activity
• receptor populations
• single molecules

• Department of Biochemistry, University at Buffalo, Buffalo, USA

  Gabriela K. Popescu

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