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Host-Bacteria Interactions

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Annette C. Vergunst

    1. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

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    You can also search for this editor in PubMed   Google Scholar

  2. David O'Callaghan

    1. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1197)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Infection Models to Study Bacterial Virulence

    1. Front Matter

      Pages 1-1
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    2. Galleria mellonella as an Infection Model for Select Agents

      • Nicolas Sprynski, Eric Valade, Fabienne Neulat-Ripoll
      Pages 3-9

    3. Drosophila as a Model for Intestinal Infections

      • Matthieu Lestradet, Kwang-Zin Lee, Dominique Ferrandon
      Pages 11-40

    4. Zebrafish Embryos as a Model to Study Bacterial Virulence

      • Jennifer Mesureur, Annette C. Vergunst
      Pages 41-66

    5. Studying Host-Pathogen Interaction Events in Living Mice Visualized in Real Time Using Biophotonic Imaging

      • Gary Splitter, Jerome Harms, Erik Petersen, Diogo Magnani, Marina Durward, Gireesh Rajashekara et al.
      Pages 67-85

    6. Intravital Two-Photon Imaging to Understand Bacterial Infections of the Mammalian Host

      • Ferdinand X. Choong, Agneta Richter-Dahlfors
      Pages 87-100

  3. Cellular Aspects of Host-Bacteria Interactions

    1. Front Matter

      Pages 101-101
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    2. Cre Reporter Assay for Translocation (CRAfT): A Tool for the Study of Protein Translocation into Host Cells

      • Amke den Dulk-Ras, Annette C. Vergunst, Paul J. J. Hooykaas
      Pages 103-121

    3. Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts with Francisella Elongation Factor Tu

      • Monique Barel, Alain Charbit
      Pages 123-139

    4. Hijacking the Host Proteasome for the Temporal Degradation of Bacterial Effectors

      • Tomoko Kubori, Andree M. Hubber, Hiroki Nagai
      Pages 141-152

    5. Live Cell Imaging of Phosphoinositide Dynamics During Legionella Infection

      • Stephen Weber, Hubert Hilbi
      Pages 153-167

    6. Investigating Interference with Apoptosis Induction by Bacterial Proteins

      • Hua Niu, Yasuko Rikihisa
      Pages 169-184

    7. Bimolecular Fluorescence Complementation for Imaging Protein Interactions in Plant Hosts of Microbial Pathogens

      • Lan-Ying Lee, Stanton B. Gelvin
      Pages 185-208

    8. Investigating TLR Signaling Responses in Murine Dendritic Cells Upon Bacterial Infection

      • Suzana Pinto Salcedo, Lena Alexopoulou
      Pages 209-225

  4. OMICS and Large Scale Screening

    1. Front Matter

      Pages 227-227
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    2. siRNA Screens Using Drosophila Cells to Identify Host Factors Required for Infection

      • Aseem Pandey, Sheng Li Ding, Thomas A. Ficht, Paul de Figueiredo
      Pages 229-244

    3. Purification of Intracellular Bacteria: Isolation of Viable Brucella abortus from Host Cells

      • Esteban Chaves-Olarte, Pamela Altamirano-Silva, Caterina Guzmán-Verri, Edgardo Moreno
      Pages 245-260

    4. RNA Sequencing of FACS-Sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens

      • Julien Rougeot, Ania Zakrzewska, Zakia Kanwal, Hans J. Jansen, Herman P. Spaink, Annemarie H. Meijer
      Pages 261-274

    5. Taking the Shortcut for High-Throughput Shotgun Proteomic Analysis of Bacteria

      • Erica Marie Hartmann, François Allain, Jean-Charles Gaillard, Olivier Pible, Jean Armengaud
      Pages 275-285
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Keywords

About this book

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.

Editors and Affiliations

  • U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

    Annette C. Vergunst, David O'Callaghan

Bibliographic Information

  • Book Title: Host-Bacteria Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Annette C. Vergunst, David O'Callaghan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1261-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1260-5Published: 29 August 2014

  • Softcover ISBN: 978-1-4939-4459-0Published: 20 September 2016

  • eBook ISBN: 978-1-4939-1261-2Published: 29 August 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 366

  • Number of Illustrations: 15 b/w illustrations, 49 illustrations in colour

  • Topics: Infectious Diseases, Bacteriology

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