Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1198)
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Table of contents (22 protocols)
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Front Matter
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Historical and Methodology Overview
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Front Matter
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Global LC-MS Based Metabolic Profiling
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Front Matter
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Global GC-MS and CE-MS Based Metabolic Profiling
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Front Matter
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Isotope Labeling Methods
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Front Matter
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Targeted Aqueous Metabolite Profiling
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Front Matter
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Lipid and Bile Acid Profiling Methods
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Front Matter
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Keywords
About this book
Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids, cells or tissue, plants, foods and related samples and this information provides enormous detail on biological systems and their current status. Mass Spectrometry in Metabolomics: Methods and Protocols presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics, this volume includes a wide variety such as triple quads, time of flight, Fourier transform ion cyclotron resonance and even simple quadrupole systems. A wide range of studies are described, with samples ranging from blood and urine to tissue and even plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Mass Spectrometry in Metabolomics: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics.
Editors and Affiliations
Bibliographic Information
Book Title: Mass Spectrometry in Metabolomics
Book Subtitle: Methods and Protocols
Editors: Daniel Raftery
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1258-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1257-5Published: 01 October 2014
Softcover ISBN: 978-1-4939-5319-6Published: 23 August 2016
eBook ISBN: 978-1-4939-1258-2Published: 01 October 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 360
Number of Illustrations: 19 b/w illustrations, 52 illustrations in colour