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Patch-Clamp Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Marzia Martina

    1. Department of Translational Bioscience, National Research Council of Canada, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Stefano Taverna

    1. Dept of Neuroscience & Brain Technologies, Italian Institute of Technology, Genoa, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1183)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. History of Electrophysiology and the Patch Clamp

    • Alexei Verkhratsky, Vladimir Parpura
    Pages 1-19

  3. Pharmacology

    1. Front Matter

      Pages 21-21
      Download chapter PDF

    2. Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications

      • Nathan G. Glasgow, Jon W. Johnson
      Pages 23-41

    3. Patch Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-Methyl-d-aspartate (NMDA) Receptor Open Channel Blockers

      • Chris G. Parsons, Kate E. Gilling
      Pages 43-63

    4. State-of-the-Art Automated Patch Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening

      • Sonja Stoelzle-Feix
      Pages 65-80

    5. Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch® 96 Using Instant Frozen Cells

      • Liudmila Polonchuk
      Pages 81-92

    6. Planar Patch Clamp for Neuronal Networks—Considerations and Future Perspectives

      • Alessandro Bosca, Marzia Martina, Christophe Py
      Pages 93-113

  4. Physiology

    1. Front Matter

      Pages 115-115
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    2. Methods for the Study of Synaptic Receptor Functional Properties

      • Enrica Maria Petrini, Andrea Barberis
      Pages 117-141

    3. Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity

      • Maria Toledo-Rodriguez, Henry Markram
      Pages 143-158

    4. Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique

      • Romain Lefebvre, Sandrine Pouvreau, Claude Collet, Bruno Allard, Vincent Jacquemond
      Pages 159-170

    5. Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability

      • Joshua L. Plotkin, D. James Surmeier
      Pages 171-182

    6. Investigation of Synaptic Microcircuits Using Patch-Clamp Paired Recordings in Acute Brain Slices

      • Giovanni Russo, Stefano Taverna
      Pages 183-193

    7. Outside-Out “Sniffer-Patch” Clamp Technique for In Situ Measures of Neurotransmitter Release

      • Émilie Muller-Chrétien
      Pages 195-204

    8. A Cost-Effective Method for Preparing, Maintaining, and Transfecting Neurons in Organotypic Slices

      • Cary Soares, Kevin F. H. Lee, Denise Cook, Jean-Claude Béïque
      Pages 205-219

    9. Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics

      • Jonathan T. Ting, Tanya L. Daigle, Qian Chen, Guoping Feng
      Pages 221-242

    10. Synaptic Connectivity in Engineered Neuronal Networks

      • Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, James J. Hickman
      Pages 243-252

    11. Modeling of Action Potential Generation in NG108-15 Cells

      • Peter Molnar, James J. Hickman
      Pages 253-261

    12. Whole-Cell Patch-Clamp Recordings in Freely Moving Animals

      • Albert K. Lee, Jérôme Epsztein, Michael Brecht
      Pages 263-276
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Keywords

About this book

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Patch-Cla

mp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

Reviews

“This is a very good up to date ‘how to’ manual with dozens of unique protocols regarding single channel, whole cell, and network patch recordings. … This atlas and textbook is a good choice for ‘patchers’, neurohysiologists, biophysicists, students, fellows and ‘cell’ molecular physiologists.” (Joseph J. Grenier, Amazon.com, September, 2015)

Editors and Affiliations

  • Department of Translational Bioscience, National Research Council of Canada, Ottawa, Canada

    Marzia Martina

  • Dept of Neuroscience & Brain Technologies, Italian Institute of Technology, Genoa, Italy

    Stefano Taverna

Bibliographic Information

  • Book Title: Patch-Clamp Methods and Protocols

  • Editors: Marzia Martina, Stefano Taverna

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1096-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1095-3Published: 15 July 2014

  • Softcover ISBN: 978-1-4939-5414-8Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1096-0Published: 14 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 403

  • Number of Illustrations: 64 b/w illustrations, 41 illustrations in colour

  • Topics: Neurosciences, Human Physiology

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