Editors:
- Includes cutting-edge methods and protocols involving axon growth and regeneration
- Provides step-by-step detail essential for reproducible results in the lab
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1162)
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Table of contents (19 protocols)
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Front Matter
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Review
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Front Matter
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Monitoring Intracellular Pathways in Growth Cones
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Front Matter
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Assessing Axonal Responses to Extracellular Factors In Vitro
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Front Matter
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Axon Injury and Regeneration In Vivo
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Front Matter
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Assessing the Extent of Axon Regeneration
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Front Matter
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About this book
Axon Growth and Regeneration: Methods and Protocols brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies. After an introductory section, this detailed volume continues with sections focusing on axon growth in vitro, providing a range of protocols that can be used to examine intracellular signalling pathways, axonal responses to extracellular factors and methods for quantifying outgrowth. The next section provides protocols for inducing experimental injury in vivo as well as some highly promising protocols for promoting regeneration, which segues into the final section highlighting a series of protocols that can be used to monitor the extent of axon regeneration in vivo, ranging from tract tracing to in vivo imaging and functional recovery. As a book in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and reliable, Axon Growth and Regeneration: Methods and Protocols aims to serve researchers studying axon regeneration with a significant set of diverse tools, vital for moving on to the next generation of exciting new discoveries in the field.
Reviews
From the reviews:
“This is a ‘how to’ manual of protocols, parameters, and cell biology methods for research studies of neurite outgrowth assays. … The authorship is very good and interesting to neurobiologists, cell culture experts, and neurology-neurosurgery researchers. … I highly recommend this book for all neurobiologists with interests in cell and slice physiology.” (Joseph J. Grenier, Amazon.com, June, 2014)Editors and Affiliations
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Department of Biochemistry and Molecular Biophysics, Columbia University, New York, USA
Andrew J. Murray
Bibliographic Information
Book Title: Axon Growth and Regeneration
Book Subtitle: Methods and Protocols
Editors: Andrew J. Murray
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0777-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-0776-2Published: 17 May 2014
Softcover ISBN: 978-1-4939-4584-9Published: 17 September 2016
eBook ISBN: 978-1-4939-0777-9Published: 16 May 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 253
Number of Illustrations: 13 b/w illustrations, 53 illustrations in colour
Topics: Neurosciences, Neurobiology