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DNA Vaccines

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Monica Rinaldi

    1. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

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  2. Daniela Fioretti

    1. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

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    You can also search for this editor in PubMed   Google Scholar

  3. Sandra Iurescia

    1. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols involving DNA vaccine applications
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1143)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. DNA Vaccine Design and Enhancement

    1. Front Matter

      Pages 1-1
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    2. A Blueprint for DNA Vaccine Design

      • Sandra Iurescia, Daniela Fioretti, Monica Rinaldi
      Pages 3-10

    3. Enhancement of Plasmid-Mediated Transgene Expression

      • Daniela Fioretti, Sandra Iurescia, Monica Rinaldi
      Pages 11-20

    4. Strategies for Improving DNA Vaccine Performance

      • Sandra Iurescia, Daniela Fioretti, Monica Rinaldi
      Pages 21-31

    5. Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies

      • Elisabete Borges Freitas, Ana Margarida Henriques, Miguel Fevereiro, Duarte Miguel Prazeres, Gabriel Amaro Monteiro
      Pages 33-59

    6. Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

      • Ronald J. Jackson, David B. Boyle, Charani Ranasinghe
      Pages 61-90

    7. Development of Antibiotic-Free Selection System for Safer DNA Vaccination

      • Jeremy M. Luke, Aaron E. Carnes, James A. Williams
      Pages 91-111

  3. Delivery System

    1. Front Matter

      Pages 113-113
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    2. Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin

      • Anita Gothelf, Julie Gehl
      Pages 115-122

    3. DNA Vaccination in Skin Enhanced by Electroporation

      • Kate E. Broderick, Amir S. Khan, Niranjan Y. Sardesai
      Pages 123-130

    4. Intradermal Vaccination by DNA Tattooing

      • Joost H. van den Berg, Koen Oosterhuis, Ton N. M. Schumacher, John B. A. G. Haanen, Adriaan D. Bins
      Pages 131-140

    5. Microneedle Applications for DNA Vaccine Delivery to the Skin

      • Hae-yong Seok, Hyemee Suh, Sunghyun Baek, Yeu-Chun Kim
      Pages 141-158

    6. Multivalent DNA-Based Vectors for DNA Vaccine Delivery

      • Young Hoon Roh, Kwang Lee, Jessica Jane Ye, Dan Luo
      Pages 159-179

    7. Superparamagnetic Nanoparticle Delivery of DNA Vaccine

      • Fatin Nawwab Al-Deen, Cordelia Selomulya, Charles Ma, Ross L. Coppel
      Pages 181-194

  4. Production, Purification, and Quality

    1. Front Matter

      Pages 195-195
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    2. Plasmid Fermentation Process for DNA Immunization Applications

      • Aaron E. Carnes, James A. Williams
      Pages 197-217

    3. Pharmaceutical Grade Large-Scale Plasmid DNA Manufacturing Process

      • Marco Schmeer, Martin Schleef
      Pages 219-240

  5. New Vaccine Applications

    1. Front Matter

      Pages 241-241
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    2. Protective and Therapeutic DNA Vaccination Against Allergic Diseases

      • Almedina Isakovic, Richard Weiss, Josef Thalhamer, Sandra Scheiblhofer
      Pages 243-258
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Keywords

About this book

DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.  Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.  Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.

Editors and Affiliations

  • Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

    Monica Rinaldi, Daniela Fioretti, Sandra Iurescia

Bibliographic Information

  • Book Title: DNA Vaccines

  • Book Subtitle: Methods and Protocols

  • Editors: Monica Rinaldi, Daniela Fioretti, Sandra Iurescia

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0410-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0409-9Published: 09 April 2014

  • Softcover ISBN: 978-1-4939-4653-2Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0410-5Published: 08 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 308

  • Number of Illustrations: 11 b/w illustrations, 32 illustrations in colour

  • Topics: Immunology, Genetic Engineering, Vaccine

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