Handbook of Biological Confocal Microscopy

1. Front Matter

   Pages i-xiii

   PDF

2. Foundations of Confocal Scanned Imaging in Light Microscopy

   • Shinya Inoué

   Pages 1-14

3. Fundamental Limits in Confocal Microscopy

   • James Pawley

   Pages 15-26

4. Quantitative Fluorescence Imaging with Laser Scanning Confocal Microscopy

   • K. Sam Wells, David R. Sandison, James Strickler, Watt W. Webb

   Pages 27-39

5. The Pixelated Image

   • Robert H. Webb, C. Kathleen Dorey

   Pages 41-51

6. Laser Sources for Confocal Microscopy

   • Enrico Gratton, Martin J. VandeVen

   Pages 53-67

7. Non-laser Illumination for Confocal Microscopy

   • Victor Chen

   Pages 69-76

8. Objective Lenses For Confocal Microscopy

   • H. Ernst Keller

   Pages 77-86

9. Size and Shape of The Confocal Spot: Control and Relation to 3D Imaging and Image Processing

   • G. J. Brakenhoff, K. Visscher, H. T. M. van der Voort

   Pages 87-91

10. The Intermediate Optical System of Laser-scanning Confocal Microscopes

    • Ernst H. K. Stelzer

    Pages 93-103

11. Intermediate Optics in Nipkow Disk Microscopes

    • G. S. Kino

    Pages 105-111

12. The Role of the Pinhole in Confocal Imaging Systems

    • T. Wilson

    Pages 113-126

13. Photon Detectors for Confocal Microscopy

    • Jonathan Art

    Pages 127-139

14. Manipulation, Display, and Analysis of Three-Dimensional Biological Images

    • Hans Chen, John W. Sedat, David A. Agard

    Pages 141-150

15. Three-dimensional Imaging on Confocal and Wide-field Microscopes

    • Walter A. Carrington, Kevin E. Fogarty, Larry Lifschitz, Fredric S. Fay

    Pages 151-161

16. Direct Recording of Stereoscopic Pairs Obtained From Disk-scanning Confocal Light Microscopes

    • Alan Boyde

    Pages 163-168

17. Fluorophores for Confocal Microscopy: Photophysics and Photochemistry

    • Roger Y. Tsien, Alan Waggoner

    Pages 169-178

18. Image Contrast in Confocal Light Microscopy

    • P. C. Cheng, R. G. Summers

    Pages 179-195

19. Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy

    • Robert Bacallao, Morgane Bomsel, Ernst H. K. Stelzer, Jan De Mey

    Pages 197-205

20. A Comparison of Various Optical Sectioning Methods: The Scanning Slit Confocal Microscope

    • Charles J. Koester

    Pages 207-214

In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim­ special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech­ and for the publication of extended abstracts.

• biology
• cell
• development
• electron microscopy
• microscopy
• neurobiology
• organizations
• physiology

• Integrated Microscopy Resource for Biomedical Research, University of Wisconsin-Madison, Madison, USA

  James B. Pawley

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