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Handbook for Rhizobia

Methods in Legume-Rhizobium Technology

  • Book
  • © 1994

Overview

Authors:
  1. Padma Somasegaran

    1. University of Hawaii NifTAL Project, Paia, USA

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  2. Heinz J. Hoben

    1. University of Hawaii NifTAL Project, Paia, USA

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    You can also search for this author in PubMed   Google Scholar

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Table of contents (38 chapters)

  1. Front Matter

    Pages i-xvi
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  2. General Microbiology of Rhizobia

    1. Front Matter

      Pages 1-6
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    2. Collecting Nodules and Isolating Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 7-23

    3. Observing the Infection Process

      • Padma Somasegaran, Heinz J. Hoben
      Pages 24-30

    4. Cultural Properties, Cell Morphology, and Nutritional Requirements of Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 31-37

    5. Demonstrating Genetic Diversity in Rhizobia Using Patterns of Carbohydrate Utilization and Intrinsic Antibiotic Resistance

      • Padma Somasegaran, Heinz J. Hoben
      Pages 38-46

    6. Quantifying the Growth of Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 47-57

    7. Counting Rhizobia by a Plant Infection Method

      • Padma Somasegaran, Heinz J. Hoben
      Pages 58-64

    8. Counting Serologically Specific Rhizobia in Soil and Peat Inoculants Using Membrane Filters and Immunofluorescence

      • Padma Somasegaran, Heinz J. Hoben
      Pages 65-74

  3. Back Matter

    Pages 75-77
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  4. Identification of Rhizobia

    1. Front Matter

      Pages 79-88
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    2. Developing Antisera

      • Padma Somasegaran, Heinz J. Hoben
      Pages 89-93

    3. Somatic Agglutination Reactions with Pure Cultures of Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 94-101

    4. Agglutinating Antigens from Root Nodules

      • Padma Somasegaran, Heinz J. Hoben
      Pages 102-106

    5. Performing Rhizobial Antigen-Antibody Reactions by Gel Immunodiffusion

      • Padma Somasegaran, Heinz J. Hoben
      Pages 107-111

    6. Determining Strain Occupancy in Soybean Nodules by Gel Immunodiffusion

      • Padma Somasegaran, Heinz J. Hoben
      Pages 112-119

    7. Producing and Applying Fluorescent Antibodies

      • Padma Somasegaran, Heinz J. Hoben
      Pages 120-130

    8. Identifying Rhizobia by the Indirect Enzyme-Linked Immunosorbent Assay

      • Padma Somasegaran, Heinz J. Hoben
      Pages 131-139

    9. Identifying Rhizobia by Immunoblot

      • Padma Somasegaran, Heinz J. Hoben
      Pages 140-148

    10. Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia

      • Padma Somasegaran, Heinz J. Hoben
      Pages 149-152
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Keywords

About this book

Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.

Authors and Affiliations

  • University of Hawaii NifTAL Project, Paia, USA

    Padma Somasegaran, Heinz J. Hoben

Bibliographic Information

  • Book Title: Handbook for Rhizobia

  • Book Subtitle: Methods in Legume-Rhizobium Technology

  • Authors: Padma Somasegaran, Heinz J. Hoben

  • DOI: https://doi.org/10.1007/978-1-4613-8375-8

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag New York, Inc. 1994

  • Softcover ISBN: 978-1-4613-8377-2Published: 17 November 2011

  • eBook ISBN: 978-1-4613-8375-8Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XVI, 450

  • Topics: Agriculture, Forestry, Plant Sciences, Cell Biology, Microbiology, Biotechnology

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