Spin Labeling

1. Front Matter

   Pages i-xix

   PDF

2. Calculating Slow Motional Magnetic Resonance Spectra

   • David J. Schneider, Jack H. Freed

   Pages 1-76

3. Inhomogeneously Broadened Spin-Label Spectra

   • Barney L. Bales

   Pages 77-130

4. Saturation Transfer Spectroscopy of Spin Labels

   • M. A. Hemminga, P. A. de Jager

   Pages 131-178

5. Nitrogen-15 and Deuterium Substituted Spin Labels for Studies of Very Slow Rotational Motion

   • Albert H. Beth, Bruce H. Robinson

   Pages 179-253

6. Experimental Methods in Spin-Label Spectral Analysis

   • Derek Marsh

   Pages 255-303

7. Electron-Electron Double Resonance

   • James S. Hyde, Jim B. Feix

   Pages 305-307

8. Resolved Electron-Electron Spin-Spin Splittings in EPR Spectra

   • Gareth R. Eaton, Sandra S. Eaton

   Pages 339-397

9. Spin-Label Oximetry

   • James S. Hyde, Witold K. Subczynski

   Pages 399-425

10. Chemistry of Spin-Labeled Amino Acids and Peptides

    • Kálmán Hideg, Olga H. Hankovszky

    Pages 427-488

11. Nitroxide Radical Adducts in Biology: Chemistry, Applications, and Pitfalls

    • Carolyn Mottley, Ronald P. Mason

    Pages 489-546

12. Advantages of 15N and Deuterium Spin Probes for Biomedical Electron Paramagnetic Resonance Investigations

    • Jane H. Park, Wolfgang E. Trommer

    Pages 547-595

13. Magnetic Resonance Study of the Combining Site Structure of a Monoclonal Anti-Spin-Label Antibody

    • Jacob Anglister

    Pages 597-613

14. Approaches to the Chemical Synthesis of 15N and Deuterium Substituted Spin Labels

    • Jane H. Park, Wolfgang E. Trommer

    Pages 615-634

15. Back Matter

    Pages 635-650

    PDF

We present this special topics volume on an area which has not received thorough coverage for over 12 years. Spin Labeling: Theory and Applications represents a complete update on new theoretical aspects and applications of the spin-label method. In the "line-shape theory" sections, we are especially pleased to include an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations. Barney Bales discusses inhomogeneous broadening phenomena in detail. Several developments in techniques and interpretation in saturation transfer spectroscopy have appeared since the publica­ tion of Spin Labeling II: Theory and Applications (L. J. Berliner, ed., Academic Press, 1979). We have included an up-to-date chapter on spin-label applications by M. A. Hemminga and P. A. de Jager. By incorporating 15N and deuterium into nitroxide spin labels, several unique advantages are derived in line-shape analysis. Albert Beth and Bruce Robinson have contributed a detailed chapter on the analysis of these labels in the slow-motion regime while Jane Park and Wolfgang Trommer present the advantages for specific biochemical examples in our "applications" section. Derek Marsh's contri­ bution on spin-label spectral analysis may be regarded as a summary chapter which touches on several of the detailed spectral analysis methods described in the earlier chapters.

• ATP
• ATPase
• Amino acid
• ENDOR
• Lipid
• Monosaccharid
• Nucleotide
• Oxidation
• Peptide
• Translation
• electron spin
• enzymes
• erythrocyte
• magnetic resonance
• spectroscopy

• Ohio State University, Columbus, USA

  Lawrence J. Berliner

• Hercules Incorporated Research Center, Wilmington, USA

  Jacques Reuben

Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.

Policies and ethics