Membrane Potential Imaging in the Nervous System

1. Front Matter

   Pages i-x

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2. Historical Overview and General Methods of Membrane Potential Imaging

   • Lawrence B. Cohen

   Pages 1-11

3. Design and Use of Organic Voltage Sensitive Dyes

   • Leslie M. Loew

   Pages 13-23

4. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines

   • Marco Canepari, Marko Popovic, Kaspar Vogt, Knut Holthoff, Arthur Konnerth, Brian M. Salzberg et al.

   Pages 25-41

5. Combined Voltage and Calcium Imaging and Signal Calibration

   • Marco Canepari, Peter Saggau, Dejan Zecevic

   Pages 43-52

6. Use of Fast-Responding Voltage-Sensitive Dyes for Large-Scale Recording of Neuronal Spiking Activity with Single-Cell Resolution

   • William N. Frost, Jean Wang, Christopher J. Brandon, Caroline Moore-Kochlacs, Terrence J. Sejnowski, Evan S. Hill

   Pages 53-60

7. Monitoring Integrated Activity of Individual Neurons Using FRET-Based Voltage-Sensitive Dyes

   • Kevin L Briggman, William B. Kristan, Jesús E. González, David Kleinfeld, Roger Y. Tsien

   Pages 61-70

8. Monitoring Population Membrane Potential Signals from Neocortex

   • Xiaoying Huang, Weifeng Xu, Kentaroh Takagaki, Jian-Young Wu

   Pages 71-81

9. Monitoring Population Membrane Potential Signals During Functional Development of Neuronal Circuits in Vertebrate Embryos

   • Yoko Momose-Sato, Katsushige Sato, Kohtaro Kamino

   Pages 83-96

10. Imaging the Dynamics of Mammalian Neocortical Population Activity In Vivo

    • Amiram Grinvald, David Omer, Shmuel Naaman, Dahlia Sharon

    Pages 97-111

11. Imaging the Dynamics of Neocortical Population Activity in Behaving and Freely Moving Mammals

    • Amiram Grinvald, Carl C. H. Petersen

    Pages 113-124

12. Monitoring Membrane Voltage Using Two-Photon Excitation of Fluorescent Voltage-Sensitive Dyes

    • Jonathan A. N. Fisher, Brian M. Salzberg

    Pages 125-138

13. Random-Access Multiphoton Microscopy for Fast Three-Dimensional Imaging

    • Gaddum Duemani Reddy, Peter Saggau

    Pages 139-145

14. Second Harmonic Imaging of Membrane Potential

    • Leslie M. Loew, Aaron Lewis

    Pages 147-155

15. Genetically Encoded Protein Sensors of Membrane Potential

    • Lei Jin, Hiroki Mutoh, Thomas Knopfel, Lawrence B. Cohen, Thom Hughes, Vincent A. Pieribone et al.

    Pages 157-163

16. Back Matter

    Pages 165-168

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The book is structured in five sections, each containing several chapters written by experts and major contributors to particular topics. The volume starts with a historical perspective and fundamental principles of membrane potential imaging and continues to cover the measurement of membrane potential signals from dendrites and axons of individual neurons, measurements of the activity of many neurons with single cell resolution, monitoring of population signals from the nervous system, and concludes with the overview of new approaches to voltage-imaging. The book is targeted at all scientists interested in this mature but also rapidly expanding imaging approach.

• Biological Microscopy

“This is an excellent ‘how to’ and methods approach to single, multiple, and populational photon imaging using various dyes optically in what is now recognized as a cutting edge technique. … This is a very technical book for intermediate to advanced neuroscientists and would be useful for both the office and laboratory. … I highly recommend this book for all interested audiences.” (Joseph J. Grenier, Amazon.com, April, 2015)

• Université Joseph Fourier, Unité Inserm 836, Grenoble Institute of Neuroscience, Grenoble, France

  Marco Canepari

• Dept. Cellular &, Molecular Physiology, Yale University School of Medicine, New Haven, USA

  Dejan Zecevic

Dejan Zecevic (b. Belgrade 1948) is a Research Scientist at the Department of Cellular and Molecular Physiology, Yale University School of Medicine. He received the PhD in Biophysics from The University of Belgrade, Serbia and was trained in the laboratory of Dr Lawrence Cohen who initiated the field of voltage-sensitive dye recording. Dejan is the pioneer of intracellular voltage-sensitive dye imaging technique, a unique and a cutting edge technology for monitoring the membrane potential fluctuation in dendritic spines and fine branches. Marco (b. Milan 1970) is first class INSERM researcher (CR1) working at the Grenoble Institute of Neuroscience. He graduated in physics at the University of Genoa and received his PhD in biophysics from the International School for Advanced Studies in Trieste. He worked at the National Institute for Medical Research in London, at Yale University and at the University of Basel. Marco is expert on several optical techniques applied to neurophysiology. Marco and Dejan collaborated for a number of years using voltage-imaging and calcium imaging approaches to study mechanisms underlying synaptic plasticity.

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