Plant Propagation by Tissue Culture

1. Front Matter

   Pages I-XI

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2. Plant Tissue Culture Procedure - Background

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 1-28

3. Micropropagation: Uses and Methods

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 29-64

4. The Components of Plant Tissue Culture Media I: Macro- and Micro-Nutrients

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 65-113

5. The Components of Plant Tissue Culture Media II: Organic Additions, Osmotic and pH Effects, and Support Systems

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 115-173

6. Plant Growth Regulators I: Introduction; Auxins, their Analogues and Inhibitors

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 175-204

7. Plant Growth Regulators II: Cytokinins, their Analogues and Antagonists

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 205-226

8. Plant Growth Regulators III : Gibberellins, Ethylene, Abscisic Acid, their Analogues and Inhibitors; Miscellaneous Compounds.

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 227-281

9. Developmental Biology

   • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

   Pages 283-333

10. Somatic Embryogenesis

    • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

    Pages 335-354

11. Adventitious Regeneration

    • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

    Pages 355-401

12. Stock Plant Physiological Factors Affecting Growth and Morphogenesis

    • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

    Pages 403-422

13. Effects of the Physical Environment

    • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

    Pages 423-464

14. The Anatomy and Morphology of Tissue Cultured Plants

    • Edwin F. George, Michael A. Hall, Geert-Jan De Klerk

    Pages 465-477

15. Back Matter

    Pages 479-501

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It is now more than twenty years since the first edition of this work appeared and nearly fifteen since the second. Whilst much of the information in those editions has stood the test of time, inevitably, because of the pace of research, a new edition is clearly timely. This is true, not only because many more species have been the subject of propagation studies, but because the background to the field – with which this volume deals – has changed almost out of all recognition. In particular, our knowledge of plant development, genetics physiology, biochemistry and molecular biology has expanded exponentially – often through work on mutants of Arabidopsis – and opened up many new avenues for the plant propagator to explore. Equally, the commercial significance of plant propagation has increased significantly. As an example, in the second edition there was a single chapter on plant growth regulators – in this there are three, reflecting the fact that not only is there more information on those PGRs we recognised in 1993, but that several new ones are now known. Equally, fifteen years ago we knew little of the molecular basis of plant development e. g. flower and shoot development, in this edition it has merited a whole chapter, much of which relates to discoveries in the last decade. Because of these factors, it was felt that a different approach was required for this edition.

• Embryo
• Plant propagation
• Tissue culture
• breeding
• conservation
• genetic engineering
• morphogenesis
• morphology
• plant breeding

From the reviews of the third edition:

"This volume is organized in 13 chapters. It is an encyclopedic text for the specialists, and the number of topics is remarkable. … The volume is written at a high scientific level, and teaches us about many theoretical, practical and new aspects in the field of plant propagation in vitro. … Therefore, I believe that everyone will surely find many topics of interest in this very important handbook that can be both used for reference and read for pleasure." (Ivan Iliev, Plant Cell, Tissue and Organ Culture, Vol. 93, 2008)

• Merriott, Somerset, United Kingdom

  Edwin F. George

• Institute of Biological Sciences, University of Wales, Aberystwyth, United Kingdom

  Michael A. Hall

• Plant Research International, Wageningen, The Netherlands

  Geert-Jan De Klerk

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