Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled invitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry. The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants. This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.
Reviews
"... summarizing so many of the results obtained with TCLs, this book is an essential acquisition for the library of tissue culture researchers." (Plant Cell, Tissue and Organ Culture, 76 (2004)
Editors and Affiliations
Institute of Biology in Dalat, Dalat, Lam Dong, Vietnam
Duong Tan Nhut
University of Ho Chi Minh City, Ho Chi Mih City, Vietnam
B. Le
Institut de Biotechnologie des Plantes, Paris, France
K. Tran Thanh Van
University of Calgary, Edmonton, Canada
T. Thorpe
Bibliographic Information
Book Title: Thin Cell Layer Culture System: Regeneration and Transformation Applications
Editors: Duong Tan Nhut, B. Le, K. Tran Thanh Van, T. Thorpe
