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Exocytosis and Endocytosis

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Florence Niedergang

    1. Department of Infection, Immunity & Inflammation, Institut Cochin (Institut National de la santé et de la recherche médicale, Centre National de la Recherche Scientifique and Université de Paris), Paris, France

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  2. Nicolas Vitale

    1. Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Strasbourg, France

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  3. Stéphane Gasman

    1. Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Strasbourg, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2233)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Endocytosis

    1. Front Matter

      Pages 1-1
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    2. Stoichiometry of Receptors at the Plasma Membrane During Their Endocytosis Using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single-Molecule Tracking

      • Laura Salavessa, Nathalie Sauvonnet
      Pages 3-17

    3. Measuring Endocytosis During Proliferative Cell Quiescence

      • Claudia Hinze, Kieran McGourty, Emmanuel Boucrot
      Pages 19-42

    4. Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution in Adrenal Chromaffin Cells

      • Mara Ceridono, Sylvette Chasserot-Golaz, Nicolas Vitale, Stéphane Gasman, Stéphane Ory
      Pages 43-51

    5. Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins

      • Massiullah Shafaq-Zadah, Estelle Dransart, Ludger Johannes
      Pages 53-70

    6. High-Content Drug Discovery Screening of Endocytosis Pathways

      • David A. Cardoso, Ngoc Chau, Phillip J. Robinson
      Pages 71-91

    7. Methods for Monitoring Endocytosis in Astrocytes

      • Maja Potokar, Jernej Jorgačevski, Robert Zorec
      Pages 93-100

    8. Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans

      • Michael A. Cousin, Karen J. Smillie
      Pages 101-111

  3. Exocytosis

    1. Front Matter

      Pages 113-113
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    2. Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion

      • David E. Gordon, Amber S. Shun-Shion, Asral W. Asnawi, Andrew A. Peden
      Pages 115-129

    3. High-Throughput Screening for Insulin Secretion Modulators

      • Michael A. Kalwat
      Pages 131-138

    4. Different Approaches to Record Human Sperm Exocytosis

      • Laila Suhaiman, Karina Noel Altamirano, Alfonsina Morales, Silvia Alejandra Belmonte
      Pages 139-168

    5. Bovine Chromaffin Cells: Culture and Fluorescence Assay for Secretion

      • Tamou Thahouly, Emeline Tanguy, Juliette Raherindratsara, Marie-France Bader, Sylvette Chasserot-Golaz, Stéphane Gasman et al.
      Pages 169-179

    6. Measurement of Exocytosis in Genetically Manipulated Mast Cells

      • Ofir Klein, Nurit P. Azouz, Ronit Sagi-Eisenberg
      Pages 181-192

    7. Super-Resolution Microscopy and Particle-Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils

      • Jennifer L. Johnson, Kersi Pestonjamasp, William B. Kiosses, Sergio D. Catz
      Pages 193-202

    8. An Approach to Monitor Exocytosis in White Adipocytes

      • Ali M. Komai, Man Mohan Shrestha, Saliha Musovic, Charlotta S. Olofsson
      Pages 203-222

    9. Amperometry in Single Cells and Tissue

      • Damien J. Keating
      Pages 223-231

    10. Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells

      • Sébastien Houy, Joana S. Martins, Ralf Mohrmann, Jakob Balslev Sørensen
      Pages 233-251

    11. Retention Using Selective Hooks-Synchronized Secretion to Measure Local Exocytosis

      • Gaelle Boncompain, Lou Fourriere, Nelly Gareil, Franck Perez
      Pages 253-264
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Keywords

About this book

This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells.  Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation.


Editors and Affiliations

  • Department of Infection, Immunity & Inflammation, Institut Cochin (Institut National de la santé et de la recherche médicale, Centre National de la Recherche Scientifique and Université de Paris), Paris, France

    Florence Niedergang

  • Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Strasbourg, France

    Nicolas Vitale, Stéphane Gasman

Bibliographic Information

  • Book Title: Exocytosis and Endocytosis

  • Book Subtitle: Methods and Protocols

  • Editors: Florence Niedergang, Nicolas Vitale, Stéphane Gasman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1044-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1043-5Published: 22 November 2020

  • Softcover ISBN: 978-1-0716-1046-6Published: 22 November 2021

  • eBook ISBN: 978-1-0716-1044-2Published: 21 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 329

  • Number of Illustrations: 12 b/w illustrations, 72 illustrations in colour

  • Topics: Cell Biology, Life Sciences, general

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