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Protein Sequencing Protocols

  • Book
  • © 2003

Overview

Editors:
  1. Bryan John Smith

    1. Celltech Chiroscience, Slough, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 211)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Strategies for Handling Polypeptides on a Micro-Scale

    • Bryan John Smith, Paul Tempst
    Pages 1-18

  3. SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing

    • Bryan John Smith
    Pages 19-30

  4. Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization

    • Michael J. Dunn
    Pages 31-39

  5. Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization

    • Michael J. Dunn
    Pages 41-48

  6. Enzymatic Cleavage of Proteins

    • Bryan John Smith
    Pages 49-61

  7. Chemical Cleavage of Polypeptides

    • Bryan John Smith
    Pages 63-82

  8. Enzymatic Preparation and Isolation of Glycopeptides

    • David J. Harvey
    Pages 83-89

  9. Analytical and Micropreparative Capillary Electrophoresis of Peptides

    • Alan J. Smith
    Pages 91-99

  10. High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues

    • Christoph W. Turck
    Pages 101-109

  11. Hydrolysis of Samples for Amino Acid Analysis

    • Ian Davidson
    Pages 111-122

  12. Amino Acid Analysis

    • G. Brent Irvine, Ian Davidson
    Pages 123-131

  13. Post Column Amino Acid Analysis

    • Alan J. Smith
    Pages 133-141

  14. Amino Acid Analysis Using Pre-Column Derivatization with 6-AminoquinolylN-Hydroxysuccinimidyl Carbamate

    • Steven A. Cohen
    Pages 143-154

  15. Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection

    • Petr Jandik, Jun Cheng, Nebojsa Avdalovic
    Pages 155-167

  16. D-Amino Acid Analysis

    • Andrea Scaloni, Maurizio Simmaco, Francesco Bossa
    Pages 169-180

  17. Validation of Amino Acid Analysis Methods

    • Andrew J. Reason
    Pages 181-194

  18. Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins

    • Fiona M. Greer, Howard R. Morris
    Pages 195-210

  19. Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry

    • Darryl J. C. Pappin
    Pages 211-219

  20. Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes

    • Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, Andrej Shevchenko
    Pages 221-234
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About this book

Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. Mass spectrometry has continued its recent rapid development to find notable application in the characterization of small amounts of protein, for example, in the field of proteomics. The “traditional” chemical N-terminal sequencing is still of great value in quality assurance of the increasing number of biopharmaceuticals that are to be found in the clinic, checking processing events of recombinant proteins, and so on. It is joined in the armory of me- ods of protein analysis by such techniques as C-terminal sequencing and amino acid analysis. These methods are continually developing. The first edition of Protein Sequencing Protocols was a “snapshot” of methods in use in protein biochemistry laboratories at the time, and this, the second edition, is likewise. Methods have evolved in the intervening period, and the content of this book has similarly changed, the content of some chapters having been superceded and replaced by other approaches. Thus, in this edition, there is inclusion of approaches to validation of methods for quality assurance work, reflecting the current importance of biopharmaceuticals, and also a guide to further analysis of protein sequence information, acknowledging the importance of bioinformatics.

Reviews

"...this book would certainly benefit readers who are new to the field as well as those more experienced as it serves as a convenient collection of tried and trusted methods...the book gives valuable background and insight to the various techniques associated with protein sequencing, which are often used by those providing services from within a core facility to the wider population of life science researchers." -Proteomics

"A positive feature of this book is that the fact that minute details, which are essential for successful implementation of an analytical method are provided. This book can serve as a useful reference for laboratories which mainly utilize chemical methods for protein analysis." -Pharmaceutical Research

From Reviews of the First Edition...

"...full of valuable information..." -Biochemical Education
"...provides very useful resources for those embarking on protein identification or characterization projects..." -FEBS Letters
"...an extremely useful compendium of protein analysis protocols..." -Nahrung-Food
"...well organized and accessible..." -Journal of the American Chemical Society

"The overall result is a concise and highly informative laboratory manual aimed at both operators and users of protein analysis facilities providing an understanding of what techniques involve, how samples should be prepared and in what form and amounts they should be submitted to achieve the most successful data. This publication has certainly proved to be a valuable asset to my laboratory and I would recommend it not only to those in protein sequencing and mass spectrometry laboratories, but also anyone with protein chemistry applications and interests."-Molecular Biotechnology

Editors and Affiliations

  • Celltech Chiroscience, Slough, UK

    Bryan John Smith

Bibliographic Information

  • Book Title: Protein Sequencing Protocols

  • Editors: Bryan John Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593429

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-0-89603-975-9Published: 10 October 2002

  • Softcover ISBN: 978-1-61737-284-1Published: 10 November 2010

  • eBook ISBN: 978-1-59259-342-2Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 493

  • Topics: Biochemistry, general

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