Meningococcal Vaccines

1. Front Matter

   Pages i-xvii

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2. Meningococcal Vaccines and Vaccine Developments

   • Ian M. Feavers

   Pages 1-22

3. Immune Response and Host-Pathogen Interactions

   • Andrew J. Pollard, David Goldblatt

   Pages 23-39

4. Purification of Capsular Polysaccharide

   • Qingling Yang, Harold Jennings

   Pages 41-47

5. Protein-Polysaccharide Conjugation

   • Zhongwu Guo, Harold Jennings

   Pages 49-54

6. N-Propionylation

   • Zhongwu Guo, Harold Jennings

   Pages 55-60

7. Outer Membrane Protein Purification

   • Carmen Arigita, Wim Jiskoot, Matthijs R. Graaf, Gideon F. A. Kersten

   Pages 61-79

8. Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease

   • Carl E. Frasch, Loek van Alphen, Johan Holst, Jan T. Poolman, Einar Rosenqvist

   Pages 81-107

9. Methods for Manipulation of Transferrin-Binding Proteins

   • Leanne M. DeWinter, Anthony B. Schryvers

   Pages 109-120

10. Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins

    • Richard F. Collins, Jeremy P. Derrick

    Pages 121-128

11. Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics

    • Janet Suker, Bambos M. Charalambous

    Pages 129-143

12. Construction of porA Mutants

    • Peter van der Ley, Loek van Alphen

    Pages 145-154

13. Construction of LPS Mutants

    • Peter van der Ley, Liana Steeghs

    Pages 155-165

14. Recombinant Proteins in Vaccine Development

    • Myron Christodoulides, Keith A. Jolley, John E. Heckels

    Pages 167-180

15. Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries

    • Paul J. Brett, Ian M. Feavers, Bambos M. Charalambous

    Pages 181-197

16. Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity

    • Alexei A. Delvig, John H. Robinson, Lee Wetzler

    Pages 199-221

17. Early Experiences with Nasal Vaccines against Meningococcal Disease

    • Johan Holst, Bjørn Haneberg

    Pages 223-239

18. Animal Models for Meningococcal Disease

    • Andrew R. Gorringe, Karen M. Reddin, Pierre Voet, Jan T. Poolman

    Pages 241-254

19. Determination of Antibody Responses to Meningococcal Antigens by ELISA

    • Einar Rosenqvist, Helena Käyhty, Andrew J. Pollard

    Pages 255-273

20. Immunoblot Analysis of Sera from Patients and Vaccinees

    • Elisabeth Wedege

    Pages 275-288

Since the first recognition of outbreaks of cerebrospinal or spotted fever at the end of the nineteenth and the beginning of the twentieth centuries, the menace of the meningococcus has been high on the list of public health prio- ties. Few if any pathogens surpass the meningococcus in the rapidity and sev- ity with which it devastates previously healthy individuals. The challenge of understanding the biology of this fascinating microbe is immense, but few will doubt that successful control of meningococcal meningitis and septicemia will only transpire through the application of a body of extraordinary detailed inf- mation, including key minutiae of its molecular biology. In the first of two companion volumes, Meningococcal Vaccines, the team of experts gathered by Andy Pollard and Martin Maiden converge to p- vide an impressive accumulation of molecular tools with which to lay bare the secrets of the meningococcus. The results of this ambitious and welcome v- ume represent an exciting and much needed resource for all of us in the field.

• infectious diseases

"This volume is an impressive collection of the latest techniques in the development, evaluation and application of meningococcal vaccines. The easy-to-follow protocols each preceded by a brief introduction into the topic were provided by leading scientists in the field. Andrew Pollard and Martin Maiden did a great job in assembling these protocols into one volume - a work we all in the meningococcal field have waited for." - International Journal of Medical Microbiology.

"The strength of this text is that it provides a key reference source, enabling researchers in the field to avoid reinventing the wheel. The text also provides the basis for the development of new ideas. Each chapter takes a step-by-step approach with clearly set out notes highlighting the problem areas. All the chapters have comprehensive bibliographies, and are well structured. The text is easily accessible and is recommended to those who are seeking to critically appraise the field of meningococcal vaccines." - Epidemiology and Infection

"This book and its accompanying volume are well produced, easy to read and well edited. Andrew Pollard and Martin Maiden are to be congratulated in bringing together in these two volumes an enormous amount of information about this very important topical infection." -Molecular Biotechnology

• BC Research Institute for Children’s and Women’s Health, Vancouver, Canada

  Andrew J. Pollard

• Wellcome Trust Centre for the Epidemiology of Infectious Disease, Oxford, UK

  Martin C.J. Maiden

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