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Transmembrane Signaling Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Dafna Bar-Sagi

    1. State University of New York at Stony Brook, NY

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 84)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Overviews

    1. Front Matter

      Pages 1-1
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    2. Peptide Recognition Mechanisms of Eukaryotic Signaling Modules

      • Chi-Hon Lee, David Cowburn, John Kuriyan
      Pages 3-31

    3. Protein–Protein Interactions in Signaling Cascades

      • Bruce J. Mayer
      Pages 33-48

    4. Transmembrane Signaling by Receptor Oligomerization

      • Mark A. Lemmon, Joseph Schlessinger
      Pages 49-71

  3. Specific Topics

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases

      • Perry M. Chan, W. Todd Miller
      Pages 75-86

    3. Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries

      • Andrew B. Sparks, James E. Rider, Brian K. Kay
      Pages 87-103

    4. Selective Antagonism of Receptor Signaling Using Antisense RNA to Deplete G-Protein Subunits

      • Paul R. Albert, Stephen J. Morris
      Pages 107-122

    5. Microinjection of Antisense Oligonucleotides and Electrophysiological Recording of Whole-Cell Currents as Tools to identify Specific G-Protein Subtypes Coupling Hormone Receptors to Voltage-Gated Calcium Channels

      • Vadim E. Degtiar, Burghardt Wittig, Günter Schultz, Frank Kalkbrenner
      Pages 123-136

    6. Oocytes Microinjection Assay to Study the MAP-Kinase Cascade

      • Juan Carlos Lacal
      Pages 139-152

    7. Mammalian Cell Microinjection Assay to Study the Function of Rac and Rho

      • Anne J. Ridley
      Pages 153-160

    8. Identification and Functional Reconstitution of Effector Proteins for the GTPases Rac and CDC42HS

      • Arie Abo
      Pages 163-172

  4. Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade

    1. Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade

      • Kazuya Shimizu, Toshihisa Ohtsuka, Yoshimi Takai
      Pages 173-183

  5. Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipases C and D

    1. Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipases C and D

      • Shamshad Cockcroft
      Pages 185-197

  6. Two-Hybrid Analysis of Ras—Raf Interactions

    1. Two-Hybrid Analysis of Ras–Raf Interactions

      • Linda Van Aelst
      Pages 201-222

  7. Cloning and Mutational Analysis of the She-Phosphotyrosine Interaction/ Phosphotyrosine-Binding Domain

    1. Cloning and Mutational Analysis of the Shc-Phosphotyrosine Interaction/Phosphotyrosine-Binding Domain

      • Vijay Yajnik, Pamela Blaikie, Ben Margolis
      Pages 223-235

  8. Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins

    1. Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins

      • Tyzoon Nomanbhoy, Richard A. Cerione
      Pages 237-247

  9. Prenylation Assays for Small GTPases

    1. Prenylation Assays for Small GTPases

      • Miguel C. Seabra, Guy L. James
      Pages 251-260

  10. Analysis of Myristoylated and Palmitoylated Src Family Proteins

    1. Analysis of Myristoylated and Palmitoylated Src Family Proteins

      • Amy Wolven, Wouter van’t Hof, Marilyn D. Resh
      Pages 261-266
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About this book

Progress within any scientific discipline rests firmly on the development of methodologies that afford new ways to address unsolved questions. In the past decade, the development and application of novel technologies and approaches for structure determination, biochemical characterization, and molecular manipulation of signaling molecules have resulted in remarkable progress in the understanding of signal transduction pathways. Thus, whereas not so long ago small molecules known as second messengers were the only identified mediators of transmembrane signals, it is now clear that many (per­ haps most) signal transduction pathways operate through complex networks of protein-protein interactions. In recognition of this central mechanism, Transmembrane Signaling Pro­ tocols is largely devoted to techniques that are widely used to study molecular crosstalk between signaling molecules. The two underlying goals of the chap­ ters assembled in this volume are: to offer technical information that allows reproduction of these methods and to provide the general principles on which these are based. In addition, introductory reviews have been included to fur­ nish a comprehensive overview of recent advances in fundamental areas of cell signaling, and to relate specific techniques to the larger picture. I thank the many authors who spent time and effort to contribute to this endeavor. It is hoped that Transmembrane Signaling Protocols will serve as a resource for future progress in the rapidly expanding field of signal transduction.

Editors and Affiliations

  • State University of New York at Stony Brook, NY

    Dafna Bar-Sagi

Bibliographic Information

  • Book Title: Transmembrane Signaling Protocols

  • Editors: Dafna Bar-Sagi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896034887

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • eBook ISBN: 978-1-59259-568-6Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 328

  • Number of Illustrations: 47 b/w illustrations

  • Topics: Cell Biology

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