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Free Radical and Antioxidant Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Donald Armstrong

    1. State University of New York, Buffalo

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 108)

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Table of contents (40 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Techniques for the Measurement of Oxidative Stress

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Oxygen Consumption Methods

      • Istvan Stadler
      Pages 3-14

    3. Spin Trapping and Electron Paramagnetic Resonance Spectroscopy

      • Daniel J. Brackett, Gemma Wallis, Michael F. Wilson, Paul B. McCay
      Pages 15-25

    4. In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy

      • Gerald M. Rosen, Sovitj Pou, Howard J. Halpern
      Pages 27-35

    5. Single Photon Counting

      • Yoshinori Mizuguchi
      Pages 37-55

    6. Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence

      • Kuldip Thusu, Ehad Abdel-Rahman, Paresh Dandona
      Pages 57-62

    7. Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography

      • Yorihiro Yamamoto, Yasuhiro Kambayashi, Takako Ueda
      Pages 63-70

    8. Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid

      • Valerian E. Kagan, Vladimir B. Ritov, Yulia Y. Tyurina, Vladimir A. Tyurin
      Pages 71-87

    9. Aromatic Hydroxylation

      • Andrea Ghiselli
      Pages 89-100

    10. Simple Assay for the Level of Total Lipid Peroxides in Serum or Plasma

      • Kunio Yagi
      Pages 101-106

    11. Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma

      • Kunio Yagi
      Pages 107-110

    12. Antibodies Against Malondialdehyde-Modified Proteins

      • Yves Chancerelle, J. Mathieu, J. F. Kergonou
      Pages 111-118

    13. Oxidized LDL and Lp(a)

      • Jan Galle, Christoph Wanner
      Pages 119-130

    14. Oxidized and Unoxidized Fatty Acyl Esters

      • Odile Sergent, Josiane Cillard
      Pages 131-137

    15. Synthesis of Lipid and Cholesterol Hydroperoxide Standards

      • Donald Armstrong, Richard W. Browne
      Pages 139-145

    16. Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids By High-Pressure Liquid Chromatography

      • Richard W. Browne, Donald Armstrong
      Pages 147-155

    17. Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo

      • Kazumasa Aoyagi, Sohji Nagase, Akio Koyama, Mitsuharu Narita, Shizuo Tojo
      Pages 157-164

    18. Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC

      • Thomas M. Wengenack, J. Randall Slemmon, J. Mark Ordy
      Pages 165-180

    19. Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA

      • Thomas M. Nicotera, Sofia Bardin
      Pages 181-190
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About this book

In Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications.

Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.


Reviews

"...the value of this book lies in the effort by the editor to help collect in one volume useful protocols and methods that will give beginners, as well as veterans in the field, a place to start for study of free radicals and antioxidants." -Analytical Biochemistry

"...this is a collection of methods whose acquisition would certainly prove useful to those working in the field of oxidative damage and which should provide plenty of ideas for developments and improvements in research methods in this field."-Angew. Chem. Inst. Ed.

"...will be a considerable help to all experimenters, especially those seeking to explore a complex system and identify the species coming into play from the initial stage of oxidants stress, i.e. the generation of the primary ROS, to the formation of secondary radicals and intermediates, the action and regulation of defense systems, and eventually to the termination of the oxidative reaction....I can but highly recommend the book to all - beginners and experienced lab hands alike - who work in the field of oxidative stress, ROS and antioxidants." - Folia Microbiologica

Editors and Affiliations

  • State University of New York, Buffalo

    Donald Armstrong

Bibliographic Information

  • Book Title: Free Radical and Antioxidant Protocols

  • Editors: Donald Armstrong

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896034720

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • eBook ISBN: 978-1-59259-254-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 455

  • Topics: Biochemistry, general

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