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The Nucleic Acid Protocols Handbook

  • Book
  • © 2000

Overview

Editors:
  1. Ralph Rapley

    1. University of Hertfordshire, Hatfield, UK

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Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (120 protocols)

  1. Front Matter

    Pages i-xxii
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  2. Nucleic Acid Extraction

    1. Front Matter

      Pages 1-1
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    2. Isolation of High-Molecular-Weight DNA from Animal Cells

      • Ian Garner
      Pages 3-7

    3. Isolation of mRNA by Affinity Chromatography

      • Sian Bryant, David L. Manning
      Pages 9-11

    4. Isolation and Purification of DNA from Plants

      • Justin Stacey, Peter G. Isaac
      Pages 13-16

    5. Purification of Uncontaminated, Intact Plant RNA

      • Shu-Hua Cheng, Brandon D. Moore, Jeffrey R. Seemann
      Pages 17-22

    6. An Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue

      • Fei Ye, Ralf Reski
      Pages 23-27

    7. Isolating Chromosomal DNA from Bacteria

      • Elisabeth Chachaty, Patrick Saulnier
      Pages 29-32

    8. Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis

      • Elisabeth Chachaty, Patrick Saulnier
      Pages 33-36

    9. Isolation of Fungal Nucleic Acids

      • Surapareddy Sreenivasaprasad
      Pages 37-45

    10. Total RNA Isolation from Bacteria

      • John Heptinstall
      Pages 47-52

    11. Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria

      • Udo Döbbeling
      Pages 53-56

    12. Spectrophotometric Analysis of Nucleic Acids

      • John Heptinstall, Ralph Rapley
      Pages 57-60

  3. Basic Separation and Analysis of DNA

    1. Front Matter

      Pages 61-61
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    2. Restriction Endonuclease Digestion of DNA

      • Duncan R. Smith
      Pages 63-66

    3. Agarose Gel Electrophoresis of Nucleic Acids

      • D. Ross Williams, Ralph Rapley
      Pages 67-70

    4. Preparation of RNA Dot Blots

      • Rachel Hodge
      Pages 71-72

    5. Native Polyacrylamide Gel Electrophoresis

      • Adrian J. Harwood
      Pages 73-75

    6. Southern Blotting of Agarose Gels by Capillary Transfer

      • Ralph Rapley, Jane Davenport-Jones
      Pages 77-79

    7. Pulsed-Field Gel Electrophoresis

      • John Maule
      Pages 81-104
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Keywords

About this book

The Nucleic Acid Protocols Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology™ series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Protocols Handbook, Ralph Rapley has brought together thewidest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect.

Reviews

"... the techniques are presented in minute details and in a "cookbook-standardized manner"... The personal notes inserted by each contributor provide assurance that the techniques would work in any laboratory... Having all of those protocols updated and tested in practice and bound in one volume will be attractive to many scientists who deal with molecular biology techniques."- Modern Pathology

"There were over 190 authors involved in writing the protocols, ...each protocol is presented in outline format an follows a common numbering scheme, making it easy to find relevant sections....Many of the protocols include tables of data or figures, including drawings or photographs of gels...One of the most useful sections in each protocol lists notes, which elucidate possible pitfalls, provide troubleshooting tips, or give alternate methods or explanations for steps. This very practical information makes successful duplication of the procedure much more likely than when attemptingto follow the often less detailed or less flexible descriptions given in journal articles....this handbook would be extremely useful as a reference in most university libraries."-E-Streams

"...it covers practically all of the very important and large field of nucleic acid research. The volume summarizes, in 10 parts, recent advances in the isolation , analysis and manipulation of nucleic acids. ...The chapters were contributed by authors from leading laboratories in the field all over the world....The book provides the reader with a large set of ready-to use protocols needed to undertake complex molecular biology experimental project on a large spectrum of biological models involving isolation and analysis of nucleic acids. It is thus an almost indispensable handbook for anybody working in the field of molecular biology and genetics."-Folia Microbiologica

"...extremely useful as a reference in most university libraries." - E-Streams

"...comprehensiveand detailed...this is an set of recipes for the genetic kitchen." -The Biologist

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    Ralph Rapley

Bibliographic Information

  • Book Title: The Nucleic Acid Protocols Handbook

  • Editors: Ralph Rapley

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1385/1592590381

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media LLC 2000

  • Hardcover ISBN: 978-0-89603-459-4Published: 24 March 2000

  • Softcover ISBN: 978-0-89603-841-7Published: 24 March 2000

  • eBook ISBN: 978-1-59259-038-4Published: 29 June 2008

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: DLXXII, 478

  • Topics: Cell Biology

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