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Forensic DNA Profiling Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Patrick J. Lincoln

    1. University of London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Jim Thomson

    1. LGC, Teddington, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 98)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Recovery of High-Molecular-Weight DNA from Blood and Forensic Specimens

    • Peter M. Schneider
    Pages 1-7

  3. Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating Resin

    • Jeanne M. Willard, Dennis A. Lee, Mitchell M. Holland
    Pages 9-18

  4. PCR Analysis of DNA from Fresh and Decomposed Bodies and Skeletal Remains in Medicolegal Death Investigations

    • Manfred N. Hochmeister
    Pages 19-26

  5. PCR Analysis from Cigaret Butts, Postage Stamps, Envelope Sealing Flaps, and Other Saliva-Stained Material

    • Manfred N. Hochmeister, Oskar Rudin, Edda Ambach
    Pages 27-32

  6. Quantification of DNA By Slot-Blot Analysis

    • Julia Andersen
    Pages 33-38

  7. Rapid Assessment of PCR Product Quality and Quantity by Capillary Electrophoresis

    • John M. Butler
    Pages 39-47

  8. Southern Blotting of Genomic DNA for DNA Profiling

    • Jim Thomson
    Pages 49-56

  9. Preparation and Use of Alkaline-Phosphatase-Conjugated Oligonucleotide Probes for Single-Locus and Multilocus VNTR Analysis

    • Wayne P. Childs, Michael B. T. Webb, Gillian Rysiecki
    Pages 57-67

  10. Preparation and Use of 32P-Labeled Single-Locus VNTR Probes in Identity Testing

    • Jürgen Henke, Lotte Henke
    Pages 69-81

  11. Statistical Methods Employed in Evaluation of Single-Locus Probe Results in Criminal Identity Cases

    • Bruce S. Weir
    Pages 83-96

  12. Interpretation and Statistical Evaluation of Multilocus DNA Fingerprints in Paternity and Relationship Testing

    • Gillian Rysiecki, John F. Y. Brookfield
    Pages 97-103

  13. Overview of PCR-Based Systems in Identity Testing

    • Bernd Brinkmann
    Pages 105-119

  14. An Introduction to PCR Primer Design and Optimization of Amplification Reactions

    • James M. Robertson, Jennifer Walsh-Weller
    Pages 121-154

  15. Analysis of Amplified Fragment-Length Polymorphisms (VNTR/STR Loci) for Human Identity Testing

    • Bruce Budowle, Robert C. Allen
    Pages 155-171

  16. Amplification of Short Tandem Repeat Loci Using PCR

    • Niels Morling
    Pages 173-180

  17. Manual Electrophoretic Methods for Genotyping Amplified STR Loci

    • Chris P. Phillips, Angel Carracedo, Maviky V. Lareu
    Pages 181-192

  18. 17 Genotyping STR Loci Using an Automated DNA Sequencer

    • David Watts
    Pages 193-208

  19. Separation of PCR Fragments by Means of Direct Blotting Electrophoresis

    • Lotte Henke, Jürgen Henke
    Pages 209-212

  20. 19 Amplification and Sequencing of Mitochondrial DNA in Forensic Casework

    • Robert J. Steighner, Mitchell Holland
    Pages 213-223
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About this book

It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis. Since that time we have witnessed a revolution in the field of forensic identification. A total change of technology, from serological or electrophoretic analysis of protein polymorphisms to direct investigation of the underlying DNA polymorphisms has occurred in a short space of time. In addition, the evolution and development of the DNA systems themselves has been rapid and spectacular. In the last decade we have progressed from the multilocus DNA fing- prints, through single locus systems based on the same Southern blot RFLP technology, to a host of systems based on the PCR technique. These include Allele Specific Oligonucleotide (ASO)-primed systems detected by dot blots, the “binary” genotypes produced by mapping variations within VNTR repeats demonstrated by minisatellite variant repeat (MVR) analysis, and yet other fragment-length polymorphisms in the form of Short Tandem Repeat (STR) loci. Hand in hand with the increasing range of systems available has been the development of new instrumentation to facilitate their analysis and allow us to explore the possibilities of high volume testing in the form of mass scre- ing and offender databases.

Reviews

"For the first time the major laboratory procedures used in human identification are brought together in one book. . .contains much valuable information."-Journal of the Forensic Science Society

Editors and Affiliations

  • University of London, UK

    Patrick J. Lincoln

  • LGC, Teddington, UK

    Jim Thomson

Bibliographic Information

  • Book Title: Forensic DNA Profiling Protocols

  • Editors: Patrick J. Lincoln, Jim Thomson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896034437

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-443-3Published: 22 January 1998

  • Softcover ISBN: 978-1-61737-038-0Published: 26 May 2011

  • eBook ISBN: 978-1-59259-204-3Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 309

  • Topics: Cell Biology

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