Basic DNA and RNA Protocols

Front Matter

The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells

• Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau

Restriction Endonuclease Digestion of DNA

• Duncan R. Smith

Agarose Gel Electrophoresis

• Duncan R. Smith

Capillary Blotting of Agarose Gels

• Duncan R. Smith, David Murphy

Random Primed 32P-Labeling of DNA

• Duncan R. Smith

Hybridization and Competition Hybridization of Southern Blots

• Rosemary E. Kelsell

Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations

• Tim Helentjaris, Tom McCreery

The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence

• Martin Cunningham, Martin Harris

Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay

• Martin Cunningham

The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence

• Bronwen Harvey, Ian Durrant, Martin Cunningham

3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection

• Martin Cunningham, Ian Durrant, Bronwen Harvey

The Preparation of Riboprobes

• Dominique Belin

Native Polyacrylamide Gel Electrophoresis

• Adrian J. Harwood

Use of Silver Staining to Detect Nucleic Acids

• Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril

End-Labeling of DNA Fragments

• Adrian J. Harwood

Northern Blot Analysis

• Robb Krumlauf

RNA Slot Blotting

• David Murphy

The RNase Protection Assay

• Dominique Belin