Overview
- Editors:
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Ralph Rapley
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School of Natural Sciences, Coventry University, Coventry, UK
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Table of contents (26 protocols)
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- Frank C. Brosius III, Lawrence B. Holzman, Xinan Cao
Pages 11-21
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- Georges-Raoul Mazars, Charles Theillet
Pages 35-40
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- Bentley A. Atchison, Andrea M. Douglas
Pages 49-55
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- Joakim Lundeberg, Bertil Pettersson, Mathias Uhlén
Pages 57-66
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- Anu Suomalainen, Ann-Christine Syvänen
Pages 67-72
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- Anu Suomalainen, Ann-Christine Syvänen
Pages 73-79
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- Siegfried Kösel, Christoph B. Lücking, Rupert Egensperger, Manuel B. Graeber
Pages 81-89
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- Harald Petry, Barbara Bachmann, Wolfgang Lüke, Gerhard Hunsmann
Pages 105-109
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- Zhiyuan Shen, Jingmei Liu, Robert L. Wells, Mortimer M. Elkind
Pages 111-118
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- Jean-Pierre Quivy, Peter B. Becker
Pages 119-131
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- Bernhard Kaltenboeck, Konstantin G. Kousoulas
Pages 149-153
About this book
Advances in bioscience research usually arise as a result of the continu ing refinement of existing technologies. However, there are a number of occa sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac tion. This technique, first reported in 1985 by MuUis and his colleagues, pro vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.
Reviews
...this is a good collection of well presented PCR protocols that should find its way into many laboratories...if you are planning on performing a large amount of PCR sequencing, or have been having problems sequencing PCR products, this book has a procedure or advice that will help. -FEBS Letters
"The book is a must for investigators in molecular biology and involved with sequencing and setting up PCR protocols....I highly recommend this most comprehensive volume for all molecular biologists."- SMG Quarterly
Editors and Affiliations
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School of Natural Sciences, Coventry University, Coventry, UK
Ralph Rapley
