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Electroporation Protocols for Microorganisms

  • Book
  • © 1995

Overview

Editors:
  1. Jac A. Nickoloff

    1. Department of Cancer Biology, Harvard University School of Public Health, Boston

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Part of the book series: Methods in Molecular Biology (MIMB, volume 47)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. Electroporation Theory

    • James C. Weaver
    Pages 1-26

  3. Instrumentation

    • Gunter A. Hofmann
    Pages 27-45

  4. Direct Plasmid Transfer Between Bacterial Species and Electrocuring

    • Helen L. Withers
    Pages 47-54

  5. Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation

    • Laura Gunn, Jennifer Whelden, Jac A. Nickoloff
    Pages 55-66

  6. Production of cDNA Libraries by Electroporation

    • Christian E. Gruber
    Pages 67-79

  7. Electroporation of RNA into Saccharomyces cerevisiae

    • Daniel R. Gallie
    Pages 81-91

  8. Electrofusion of Yeast Protoplasts

    • Herbert Weber, Hermann Berg
    Pages 93-104

  9. Escherichia coli Electrotransformation

    • Elizabeth M. Miller, Jac A. Nickoloff
    Pages 105-113

  10. Electrotransformation in Salmonella

    • Kenneth E. Sanderson, P. Ronald MacLachlan, Andrew Hessel
    Pages 115-123

  11. Electrotransformation of Pseudomonas

    • Jonathan J. Dennis, Pamela A. Sokol
    Pages 125-133

  12. Electroporation of Xanthomonas

    • Teresa J. White, Carlos F. Gonzalez
    Pages 135-141

  13. Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids

    • John R. McQuiston, Gerhardt G. Schurig, Nammalwar Sriranganathan, Stephen M. Boyle
    Pages 143-148

  14. Electroporation of Francisella tularensis

    • Gerald S. Baron, Svetlana V. Myltseva, Francis E. Nano
    Pages 149-154

  15. A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation

    • Hajime Hamashima, Makoto Iwasaki, Taketoshi Arai
    Pages 155-160

  16. Genetic Transformation of Bacteroides spp. Using Electroporation

    • C. Jeffrey Smith
    Pages 161-169

  17. Electrotransformation of Agrobacterium

    • Jhy-Jhu Lin
    Pages 171-178

  18. Electroporation of Helicobacter pylori

    • Ellyn D. Segal
    Pages 179-184

  19. Electrotransformation of Streptococci

    • Robert E. McLaughlin, Joseph J. Ferretti
    Pages 185-193

  20. Transformation of Lactococcus by Electroporation

    • Helge Holo, Ingolf F. Nes
    Pages 195-199
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About this book

Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro­ organisms describe the underlying theory of electroporation, the com­ mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre­ sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro­ duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ­ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.

Reviews

...the most comprehensive treatment available on the topic of electroporation of microorganisms...will be a useful resource for scientists working on diverse aspects of microbial genetics.-Four Stars, Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • Department of Cancer Biology, Harvard University School of Public Health, Boston

    Jac A. Nickoloff

Bibliographic Information

  • Book Title: Electroporation Protocols for Microorganisms

  • Editors: Jac A. Nickoloff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033104

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1995

  • Softcover ISBN: 978-0-89603-310-8Published: 20 June 1995

  • eBook ISBN: 978-1-59259-534-1Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 372

  • Topics: Cell Biology

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