Transgenesis Techniques

1. Front Matter

   Pages i-xii

   PDF

2. Introduction and Topical Reviews

   1. Introduction to Transgenesis

      • David Murphy, David A. Carter

      Pages 3-5

   2. Transgenic Rodents and the Study of the Central Nervous System

      • David A. Carter

      Pages 7-22

   3. Transgenic Animals and the Study of Cancer

      • David Murphy

      Pages 23-36

   4. Transgenic Animals and the Study of the Immune System

      • Kam M. Hui

      Pages 37-52

   5. The Commercial and Agricultural Applications of Animal Transgenesis

      • Kevin A. Ward, Colin D. Nancarrow

      Pages 53-71

3. Transgenesis in Invertebrate and Lower Vertebrate Species

   1. P Element-Mediated Germ-Line Transformation of Drosophila

      • Mark O’Connor, William Chia

      Pages 75-85

   2. Gene Transfer by Microinjection in the Zebra Fish Brachydanio rerio

      • Hong-Woo Khoo, Lay-Hong Ang, Hueh-Bin Lim

      Pages 87-94

   3. Transgenic Induction in Salmonid and Tilapia Fish

      • Norman Maclean

      Pages 95-107

4. Transgenesis in the Mouse

   1. An Overview of Transgenic Mouse Production

      • David Murphy, David A. Carter

      Pages 111-114

   2. Transgene Design

      • David Murphy, David A. Carter, Duncan R. Smith

      Pages 115-118

   3. Isolation of DNA Fragments for Microinjection

      • Duncan R. Smith, David Murphy

      Pages 119-123

   4. Establishing a Colony for Efficient Production of Transgenic Mice

      • David A. Carter

      Pages 125-129

   5. Mating Mice

      • David Murphy

      Pages 131-134

   6. Anesthetizing Mice

      • David A. Carter

      Pages 135-136

   7. Vasectomizing a Mouse

      • David Murphy

      Pages 137-140

   8. Preparation of Culture Media for Fertilized One-Cell Mouse Eggs

      • David A. Carter

      Pages 141-143

   9. Collection of Fertilized One-Cell Mouse Eggs for Microinjection

      • David A. Carter

      Pages 145-149

   10. Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs

       • David Murphy

       Pages 151-162

   11. Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs

       • David Murphy

       Pages 163-167

Over the past twenty years, the disciplines of cellular and molecular biology have generated a bounty of information pertaining to the understanding of the structure and function of eukaryotic cells . It is now recognized that individual cells regulate their activities in response to a multitude of external signals ; that external signals, a- ing through a limited number of second messenger systems, modulate the cell's pattern of gene expression; and that the cohort of genes available for regulation is dependent upon the developmental history of the cell. Extrapolating these truths to the whole organism is a challenge that has been met by an increasing number of investigators . The wealth of information that molecular and cell biology has provided us with is now being integrated into an understanding of whole t- sues, organs, and organisms. Without such an integration, the molecular information will be nothing more than a confusing catalog of sequences and structures. Central to this project will be the app- cation of the powerful new techniques described in Transgenesis Techniques: Principles and Protocols .

Such a book is precious...a must in every laboratory...-Cellular and Molecular Biology

• National University of Singapore, Republic of Singapore

  David Murphy, David A. Carter

Policies and ethics