Human Cell Culture

1. Front Matter

   Pages i-vi

   PDF

2. Hematopoietic Stem and Progenitor Cells

   • Manfred R. Koller, Bernhard 0. Palsson

   Pages 1-30

3. In Vitro T-Lymphopoiesis

   • Michael Rosenzweig, David T Scadden

   Pages 31-44

4. T-Lymphocytes: Mature Polyclonal and Antigen-Specific Cell Culture

   • Bruce L Levine, Katia Schlienger, Carl H June

   Pages 45-100

5. The Culture, Characterization and Triggering of B-Lymphocytes

   • Gerrard Teoh, Kenneth C Anderson

   Pages 101-124

6. Monocytes and Macrophages

   • Ivan N Rich

   Pages 125-145

7. Isolation and Cultivation of Osteoclasts and Osteoclast-Like Cells

   • Philip Osdoby, Fred Anderson, William Maloney, Patricia Collin-Osdoby

   Pages 147-169

8. Isolation and Culture of Dendritic Cells

   • Michael A Morse, H Kim Lyerly

   Pages 171-191

9. In Vitro Proliferation and Differentiation of CD34+ Cells to Neutrophils

   • James G Bender

   Pages 193-217

10. Isolation and Culture of Eosinophils

    • Helene F Rosenberg

    Pages 219-239

11. Isolation and Culture of Mast Cells and Basophils

    • Peter Valent

    Pages 241-257

12. Purification and Culture of Erythroid Progenitor Cells

    • Chun-Hua Dai, Amittha Wickrema, Krantz Sanford B

    Pages 259-286

13. In Vitro Development of Megakaryocytes and Platelets

    • Marcus 0 Muench, Jae-Hung Shieh

    Pages 287-315

14. Perspectives, Ethics and Clinical Issues in the Use of Primary Human Cells

    • Mary Pat Moyer

    Pages 317-342

The daily production of hundreds of billions of blood cells through the process of hematopoiesis is a remarkable feat of human physiology. Transport of oxygen to tissues, blood clotting, antibody- and cellular-mediated immunity, bone remodeling, and a host of other functions in the body are dependent on a properly functioning hematopoietic system. As a consequence, many pathological conditions are attributable to blood cell abnormalities, and a fair number of these are now clinically treatable as a direct result of hematopoietic research. Proliferation of hematopoietic stem cells, and their differentiation into the many different lineages of functional mature cells, is highly regulated and responsive to many environmental and physiological challenges. Our relatively advanced understanding of this stem cell system provides potentially important insights into the regulation of development in other tissues, many of which are now being acknowledged as stem ce- based, perhaps even into adulthood. The recent public and scientific fanfare following announcement of human embryonic stem cell studies suggests that stem cell research will continue to be a relevant and exciting topic.

• Macrophages
• bioengineering
• development
• neutrophils
• platelets
• tissue

• Oncosis, San Diego, USA

  Manfred R. Koller

• Department of Bioengineering, University of California, La Jolla, USA

  Bernhard O. Palsson

• Institute of Urolog, UniversityCollegeLondon, London, UK

  John R. W. Masters

Policies and ethics