Overview
- Comprehensively summarises the different approaches to assess drug absorption from in vitro models
- Gives a review on the current state-of-the-art in pharmaceutical cell biology
- Provides readers with helpful protocols for many applications that can be directly used at the laboratory bench
Part of the book series: Biotechnology: Pharmaceutical Aspects (PHARMASP, volume VII)
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Table of contents (28 chapters)
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Front Matter
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Perfused Organ Level/In Situ Techniques
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Cellular Level—In Vitro Models of Epithelial and Endothelial Barriers
Keywords
About this book
In the last 15 years, a great number of cell- or tissue-based in vitro models have been introduced into the biopharmaceutics arena. These models mimic the different biological barriers that a drug has to overcome to finally reach its target organ/cell/receptor. These in vitro models have been found very useful in not only characterising the permeability behaviour of drugs molecules in epithelial and endothelial tissues, but also studying drug delivery systems for improved delivery and enhanced absorption. Compared to the complex in vivo situation, in vitro models offer a fast, convenient approach with cost advantages most of times. Most importantly, they can be standardised and automatised to be applicable to the high-throughput screening.
Starting at the molecular level of studies, continuing with cell monolayer models (both primary and cell lines) and in situ techniques as a final testing format, the book provides a practical approach to contemporary in vitro techniques for drug absorption studies. In addition, chapters on high-throughput assays, in vitro-in vivo correlation, bioinformatics and regulatory issues are covered, giving a comprehensive overview of available models and techniques. Moreover, an appendix comprised with a number of practical protocols is available online, updated as needed, should prove very helpful to apply the techniques directly to the benchside.
Editors and Affiliations
Bibliographic Information
Book Title: Drug Absorption Studies
Book Subtitle: In Situ, In Vitro and In Silico Models
Editors: Carsten Ehrhardt, Kwang-Jin Kim
Series Title: Biotechnology: Pharmaceutical Aspects
DOI: https://doi.org/10.1007/978-0-387-74901-3
Publisher: Springer New York, NY
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer-Verlag US 2008
Hardcover ISBN: 978-0-387-74900-6Published: 18 January 2008
Softcover ISBN: 978-1-4899-7754-0Published: 23 August 2016
eBook ISBN: 978-0-387-74901-3Published: 22 December 2007
Edition Number: 1
Number of Pages: XXII, 696
Topics: Pharmacology/Toxicology, Biotechnology, Cell Biology, Medicine/Public Health, general
