Overview
- Authors:
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Michael J. Dykstra
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North Carolina State University, USA
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Laura E. Reuss
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North Carolina State University, USA
Covers a wide range of topics including conventional light microscopy, transmission electron microscopy, and scanning electron microscopy
Includes a survey of each subject, and a techniques section, where appropriate, with tried-and-true methods that will produce publishable results
Serves as a broadly applicable starting point for a variety of approaches to cytological research
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Table of contents (32 chapters)
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Front Matter
Pages i-xviii
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- Michael J. Dykstra, Laura E. Reuss
Pages 1-73
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- Michael J. Dykstra, Laura E. Reuss
Pages 74-123
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- Michael J. Dykstra, Laura E. Reuss
Pages 125-149
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- Michael J. Dykstra, Laura E. Reuss
Pages 150-152
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- Michael J. Dykstra, Laura E. Reuss
Pages 153-158
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- Michael J. Dykstra, Laura E. Reuss
Pages 159-174
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- Michael J. Dykstra, Laura E. Reuss
Pages 175-189
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- Michael J. Dykstra, Laura E. Reuss
Pages 190-196
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- Michael J. Dykstra, Laura E. Reuss
Pages 197-208
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- Michael J. Dykstra, Laura E. Reuss
Pages 209-217
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- Michael J. Dykstra, Laura E. Reuss
Pages 219-231
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- Michael J. Dykstra, Laura E. Reuss
Pages 232-239
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- Michael J. Dykstra, Laura E. Reuss
Pages 241-244
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- Michael J. Dykstra, Laura E. Reuss
Pages 245-258
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- Michael J. Dykstra, Laura E. Reuss
Pages 259-270
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- Michael J. Dykstra, Laura E. Reuss
Pages 271-285
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- Michael J. Dykstra, Laura E. Reuss
Pages 287-322
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- Michael J. Dykstra, Laura E. Reuss
Pages 323-337
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- Michael J. Dykstra, Laura E. Reuss
Pages 339-345
About this book
Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
Reviews
"In this second edition of his 1992 hardcover text and 1993 spiral-bound lab manual on Biological Electron Microscopy, Michael Dykstra has expended considerable effort to merge the two earlier volumes into a more readable and usable single volume and also to update them and add considerable new material. Happily, the result is a first rate, comprehensive book that will be useful for both teaching beginning students and as a reference book for experienced researchers.
In all chapters, new materials have been added in the text and referenced at the chapter ends, and where appropriate, useful web sites have been indicated where additional information may be obtained. All of the excellent illustrative photographs from the first edition have been retained. As before, the chapters on microscope construction and operation are very well done and will be invaluable in teaching. Cautionary statements are made throughout about handling and disposal of the hazardous materials used in EM labs. Relevant journals, societies, and equipment suppliers are listed in appendices.
In general, this single volume is a welcome addition to the literature available on biological microscopy. Its publication offers support for the idea that microscopy in all of its guises is still a dynamic and valuable tool for all biologists."
(Henry C. Aldrich, Professor Emeritus of Microbiology and Cell Science, University of Florida, Gainesville)