Introduction to Cell and Tissue Culture

1. Front Matter

   Pages i-xv

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2. Introduction

   Pages 1-8

3. Setting Up a Cell Culture Laboratory

   Pages 9-23

4. The Physical Environment

   Pages 25-40

5. Media

   Pages 41-62

6. Standard Cell Culture Techniques

   Pages 63-87

7. Looking at Cells

   Pages 89-115

8. Contamination: How to Avoid It, Recognize It, and Get Rid of It

   Pages 117-127

9. Serum-Free Culture

   Pages 129-150

10. Primary Cultures

    Pages 151-163

11. Establishing a Cell Line

    Pages 165-174

12. Special Growth Conditions

    Pages 175-194

13. Cell Culture for Commercial Settings

    Pages 195-204

14. Back Matter

    Pages 205-241

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It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The­ ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought­ ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method­ ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for­ mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use­ ful sources of information. In addition to the fundamentals, attention is also given to mod­ ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot­ ed to any of the many disciplines to which cell culture methodology is applicable.

• biology
• cell biology
• cell culture
• cell lines
• endocrinology
• genetics
• physiology
• tissue

• Genentech Inc., South San Francisco

  Jennie P. Mather, Penelope E. Roberts

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